Western Blot (WB)
(Western blot analysis of Speedy A expression in PC3 (A), U87 (B) whole cell lysates.)
Rabbit Speedy A Polyclonal Antibody | anti-SPDYA antibody
Anti-Speedy A Antibody
Gene Names
SPDYA; SPY1; SPDY1; RINGO3; RINGOA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Purified by immunogen affinity chromatography
Synonyms
Speedy A; Polyclonal Antibody; Anti-Speedy A Antibody; SPDY1; SPY1; Speedy protein A; Rapid inducer of G2/M progression in oocytes A; RINGO A; hSpy/Ringo A; Speedy-1; Spy1; anti-SPDYA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Speedy A protein.
Purity/Purification
Purified by immunogen affinity chromatography
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
286
Applicable Applications for anti-SPDYA antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1/500 - 1/2000
IF/ICC: 1/50 - 1/200
IF/ICC: 1/50 - 1/200
Immunogen
Recombinant full length protein of human Speedy A
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of Speedy A expression in PC3 (A), U87 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Speedy A expression in PC3 (A), U87 (B) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Speedy A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Speedy A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Related Product Information for anti-SPDYA antibody
Rabbit polyclonal antibody to Speedy A
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,269 Da
NCBI Official Full Name
speedy protein A isoform 2
NCBI Official Synonym Full Names
speedy/RINGO cell cycle regulator family member A
NCBI Official Symbol
SPDYA
NCBI Official Synonym Symbols
SPY1; SPDY1; RINGO3; RINGOA
NCBI Protein Information
speedy protein A
UniProt Protein Name
Speedy protein A
Protein Family
UniProt Gene Name
SPDYA
UniProt Synonym Gene Names
RINGO A; hSpy/Ringo A; Spy1
Uniprot Description
Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2 (PubMed:12972555). Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation (PubMed:28666995). Mediates cell survival during the DNA damage process through activation of CDK2 (PubMed:12839962).
Research Articles on SPDYA
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Product Notes
The SPDYA spdya (Catalog #AAA8291921) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Speedy An Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Speedy A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000 IF/ICC: 1/50 - 1/200. Researchers should empirically determine the suitability of the SPDYA spdya for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Speedy A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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