Rabbit anti-Human E2F1 Polyclonal Antibody | anti-E2F1 antibody

E2F1 (S332) (Transcription Factor E2F1, E2F-1, Retinoblastoma Binding Protein 3, RBBP-3, PRB-binding Protein E2F-1, PBR3, Retinoblastoma-associated Protein 1, RBAP-1, RBBP3) (PE)

Cat. Num. AAA6473385

• Gene Names: E2F1; RBP3; E2F-1; RBAP1; RBBP3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: E2F1; Polyclonal Antibody; E2F1 (S332) (Transcription Factor E2F1; E2F-1; Retinoblastoma Binding Protein 3; RBBP-3; PRB-binding Protein E2F-1; PBR3; Retinoblastoma-associated Protein 1; RBAP-1; RBBP3) (PE); anti-E2F1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human E2F1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-E2F1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding S332 of human E2F1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-E2F1 Antibody (S332) in 293 cell line lysates (35ug/lane). E2F1 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-E2F1 antibody

Antibodies; Retinoblastoma Proteins

References

1. O'Donnell, K.A., et al., Nature 435(7043):839-843 (2005). 2. Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005). 3. Joshi, B., et al., Oncogene 24(13):2204-2217 (2005). 4. Saberwal, G., et al., Int. J. Hematol. 80(2):146-154 (2004). 5. Chaussepied, M., et al., Mol. Cell 16(5):831-837 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 12669911
• NCBI GeneID: 1869
• NCBI Accession #: NP_005216
• NCBI GenBank Nucleotide #: NM_005225
• UniProt Accession #: Q01094; Q13143; Q92768
• Molecular Weight: 46,920 Da
• NCBI Official Full Name: transcription factor E2F1
• NCBI Official Synonym Full Names: E2F transcription factor 1
• NCBI Official Symbol: E2F1
• NCBI Official Synonym Symbols: RBP3; E2F-1; RBAP1; RBBP3
• NCBI Protein Information: transcription factor E2F1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; retinoblastoma-associated protein 1; retinoblastoma-binding protein 3
• UniProt Protein Name: Transcription factor E2F1
• Protein Family: Transcription factor
• UniProt Gene Name: E2F1
• UniProt Synonym Gene Names: RBBP3; E2F-1; RBAP-1; RBBP-3
• UniProt Entry Name: E2F1_HUMAN

Uniprot Description

E2F1: a member of the E2F/DP family of transcription factors.The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Binds DNA cooperatively with Dp transcription factors in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.The Dp-1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. It can mediate both cell proliferation and p53-dependent apoptosis.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: nucleoplasm; Rb-E2F complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activity

Biological Process: Notch signaling pathway; transcription, DNA-dependent; apoptosis; positive regulation of transcription, DNA-dependent; mRNA stabilization; negative regulation of transcription from RNA polymerase II promoter; anoikis; positive regulation of fibroblast proliferation; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of transcription, DNA-dependent; DNA damage checkpoint; forebrain development; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle