Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 10% SDS PAGE gel and E2F1 antibody at a dilution of 1:500)
Rabbit E2F1 Polyclonal Antibody | anti-E2F1 antibody
E2F1 Antibody
Gene Names
E2f1; E2F-1; mKIAA4009; Tg(Wnt1-cre)2Sor
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
E2F1; Polyclonal Antibody; E2F1 Antibody; Affinity Purified Rabbit Polyclonal E2F1 Antibody; Polyclonal E2F1 antibody; Anti-E2F1 antibody; E2F-1 antibody; RBAP1 antibody; EF1 2; EF1-2; RBAP-1 antibody; EF1-2 antibody; PRB-binding protein E2F-1 antibody; RBBP3 antibody; retinoblastoma-binding protein 3 antibody; RBBP-3 antibody; transcription factor E2F1 antibody; E2F1 antibody; EF1 2 antibody; RBP3 antibody; anti-E2F1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
430
Applicable Applications for anti-E2F1 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
IF: 1:100-1:500
ICC: 1:100-1:500
WB: 1:500-1:3000
ICC: 1:100-1:500
WB: 1:500-1:3000
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 10% SDS PAGE gel and E2F1 antibody at a dilution of 1:500)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 10% SDS PAGE gel and E2F1 antibody at a dilution of 1:500)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using E2F1 antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using E2F1 antibody at 1:200 dilution.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: A431; B: H1299) using a 10% SDS PAGE gel and E2F1 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: A431; B: H1299) using a 10% SDS PAGE gel and E2F1 antibody at a dilution of 1:1000)
Related Product Information for anti-E2F1 antibody
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins, a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Product Categories/Family for anti-E2F1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
47 kDa
NCBI Official Full Name
E2F1
NCBI Official Synonym Full Names
E2F transcription factor 1
NCBI Official Symbol
E2f1
NCBI Official Synonym Symbols
E2F-1; mKIAA4009; Tg(Wnt1-cre)2Sor
NCBI Protein Information
transcription factor E2F1
Protein Family
Research Articles on E2F1
Similar Products
Product Notes
The E2F1 (Catalog #AAA5312404) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's E2F1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB). IF: 1:100-1:500 ICC: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the E2F1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E2F1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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