Western Blot (WB)
(Western Blot: Sample: Mouse Skeletal muscle lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse DAG1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Dystrophin Associated Glycoprotein 1 Polyclonal Antibody | anti-DAG1 antibody
Dystrophin Associated Glycoprotein 1 (DAG1) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Mouse Skeletal muscle lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse DAG1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Skeletal muscle lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse DAG1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. A complete lack of the encoded protein in mice results in embryonic lethality due to the disorganization of Reichert's membrane. Chimeric mice deficient in the encoded protein overcome embryonic lethality but develop a progressive muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. [provided by RefSeq, Nov 2015]
Uniprot Description
DAG1: a cytoskeletal protein that functions as a laminin receptor. Provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. May be involved in autosomal recessive muscular dystrophies. Its dramatic reduction in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. Binds to several types of arenaviruses. Is a target for the entry of Mycobacterium leprae into peripheral nerve Schwann cells. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence.
Protein type: Cytoskeletal; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 9 F1|9 59.08 cM
Cellular Component: basement membrane; basolateral plasma membrane; cell junction; cell-cell adherens junction; contractile ring; costamere; cytoplasm; cytoskeleton; dystroglycan complex; dystrophin-associated glycoprotein complex; external side of plasma membrane; extracellular exosome; extracellular matrix; extracellular region; extracellular space; filopodium; focal adhesion; integral component of membrane; intercellular junction; lamellipodium; membrane; membrane raft; nuclear periphery; nucleoplasm; nucleus; plasma membrane; postsynaptic membrane; sarcolemma; synapse
Molecular Function: actin binding; alpha-actinin binding; calcium ion binding; laminin binding; laminin-1 binding; protein binding; SH2 domain binding; structural constituent of muscle; tubulin binding; vinculin binding
Biological Process: basement membrane organization; calcium-dependent cell-matrix adhesion; commissural neuron axon guidance; cytoskeletal anchoring; membrane protein ectodomain proteolysis; morphogenesis of an epithelial sheet; myelination in the peripheral nervous system; negative regulation of cell migration; negative regulation of MAPKKK cascade; negative regulation of protein kinase B signaling cascade; nerve maturation; NLS-bearing substrate import into nucleus; positive regulation of basement membrane assembly involved in embryonic body morphogenesis; positive regulation of cell-matrix adhesion; positive regulation of myelination; positive regulation of oligodendrocyte differentiation; positive regulation of protein kinase activity; regulation of epithelial to mesenchymal transition; regulation of gastrulation; Schwann cell development; viral process; virus-host interaction
Research Articles on DAG1
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Product Notes
The DAG1 dag1 (Catalog #AAA2032517) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Dystrophin Associated Glycoprotein 1 (DAG1) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Dystrophin Associated Glycoprotein 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the DAG1 dag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dystrophin Associated Glycoprotein 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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