Rabbit anti-Human DYRK1B Polyclonal Antibody | anti-DYRK1B antibody

DYRK1B, ID (DYRK1B, MIRK, Dual specificity tyrosine-phosphorylation-regulated kinase 1B, Minibrain-related kinase, Mirk protein kinase) (Biotin)

Cat. Num. AAA6293996

• Gene Names: DYRK1B; MIRK
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DYRK1B; Polyclonal Antibody; ID (DYRK1B; MIRK; Dual specificity tyrosine-phosphorylation-regulated kinase 1B; Minibrain-related kinase; Mirk protein kinase) (Biotin); Mirk protein kinase; anti-DYRK1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DYRK1B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DYRK1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-405 amino acids from the Central region of human DYRK1B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DYRK1B Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the DYRK1B antibody detected the DYRK1B protein (arrow).)

Related Product Information for anti-DYRK1B antibody

DYRK1B is a member of the DYRK family of protein kinases. DYRK1B contains a bipartite nuclear localization signal and is found mainly in muscle and testis. The protein is proposed to be involved in the regulation of nuclear functions. Three isoforms of DYRK1B have been identified differing in the presence of two alternatively spliced exons within the catalytic domain. [provided by RefSeq].

Product Categories/Family for anti-DYRK1B antibody

Antibodies; Protein Kinases

References

Lauth, M., et al. Nat. Struct. Mol. Biol. 17(6):718-725(2010). Yang, C., et al. Carcinogenesis 31(4):552-558(2010). Jin, K., et al. J. Biol. Chem. 284(34):22916-22925(2009). Deng, X., et al. Cancer Res. 69(8):3317-3324(2009). Janumyan, Y., et al. J. Biol. Chem. 283(49):34108-34120(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4758222
• NCBI GeneID: 9149
• NCBI Accession #: NP_004705.1; NP_006474.1
• NCBI GenBank Nucleotide #: NM_004714.1
• UniProt Accession #: Q9Y463; O75258; O75788; O75789
• Molecular Weight: 69,198 Da
• NCBI Official Full Name: dual specificity tyrosine-phosphorylation-regulated kinase 1B isoform a
• NCBI Official Synonym Full Names: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B
• NCBI Official Symbol: DYRK1B
• NCBI Official Synonym Symbols: MIRK
• NCBI Protein Information: dual specificity tyrosine-phosphorylation-regulated kinase 1B; mirk protein kinase; minibrain-related kinase
• UniProt Protein Name: Dual specificity tyrosine-phosphorylation-regulated kinase 1B
• Protein Family: Dual specificity tyrosine-phosphorylation-regulated kinase
• UniProt Gene Name: DYRK1B
• UniProt Synonym Gene Names: MIRK
• UniProt Entry Name: DYR1B_HUMAN

NCBI Description

DYRK1B is a member of the DYRK family of protein kinases. DYRK1B contains a bipartite nuclear localization signal and is found mainly in muscle and testis. The protein is proposed to be involved in the regulation of nuclear functions. Three isoforms of DYRK1B have been identified differing in the presence of two alternatively spliced exons within the catalytic domain. [provided by RefSeq, Jul 2008]

Uniprot Description

DYRK1B: a dual-specificity protein kinase of the DYRK family. Localizes in the nucleus, where it enhances the transcriptional activity of TCF1/HNF1A. A co-activator of FKHR (FOXO1a)-dependent glucose-6-phosphatase gene expression. Induced by members of the Rho-family in myoblasts. Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines. Contains a bipartite nuclear targeting signal sequence. Three splice-variant isoforms have been described.

Protein type: Kinase, protein; EC 2.7.12.1; Protein kinase, CMGC; Protein kinase, dual-specificity (non-receptor); CMGC group; DYRK family; Dyrk1 subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transcription coactivator activity; protein serine/threonine/tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; myoblast fusion

Disease: Abdominal Obesity-metabolic Syndrome 3