Rabbit Dyrk1A Polyclonal Antibody | anti-Dyrk1A antibody

Dyrk1A (Q39) Polyclonal Antibody

Cat. Num. AAA3002119

• Gene Names: DYRK1A; MNB; DYRK; HP86; MNBH; MRD7; DYRK1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Dyrk1A; Polyclonal Antibody; Dyrk1A (Q39) Polyclonal Antibody; Protein kinase minibrain homolog; MNBH; HP86; Dual specificity YAK1-related kinase; Hmnb; anti-Dyrk1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Dyrk1A (Q39) polyclonal antibody detects endogenous levels of Dyrk1A protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 763

• Applicable Applications for anti-Dyrk1A antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 6-58 of Human Dyrk1A.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Dyrk1A (Q39) pAb at 1:500 dilutionLane1:Hela whole cell lysate (20ug) Lane2:MCF-7 whole cell lysate (10ug) Lane3:A549 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:MEF whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Dyrk1A (Q39) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Dyrk1A (Q39) pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Dyrk1A (Q39) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-Dyrk1A antibody

Dyrk (for dual specificity tyrosine phosphorylation regulated kinase) is the homolog of the Drosophila mnb (minibrain) gene, which is required for neurogenesis. Dyrk is a dual-specificity tyrosine kinase and serine/threonine kinase, which is itself regulated by tyrosine phosphorylation. Several mammalian Dyrk related proteins have been identified and are thought to compose a family of dual specificity protein kinases. Dyrk family members, including Dyrk1A (originally Dyrk), Dyrk1B, Dryk1C, Dyrk2, Dyrk3, Dyrk4A and Dyrk4B, are thought to be involved in diverse cellular functions. Dyrk1A is a candidate gene that may be involved in Downs syndrome, and it has been found to be somewhat overexpressed in Downs syndrome.

NCBI and Uniprot Product Information

• NCBI GI #: 18765758
• NCBI GeneID: 1859
• NCBI Accession #: NP_001387.2
• NCBI GenBank Nucleotide #: NP_001387.2
• UniProt Accession #: Q13627
• Molecular Weight: ~ 90kDa
• NCBI Official Full Name: dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform 1
• NCBI Official Synonym Full Names: dual specificity tyrosine phosphorylation regulated kinase 1A
• NCBI Official Symbol: DYRK1A
• NCBI Official Synonym Symbols: MNB; DYRK; HP86; MNBH; MRD7; DYRK1
• NCBI Protein Information: dual specificity tyrosine-phosphorylation-regulated kinase 1A
• UniProt Protein Name: Dual specificity tyrosine-phosphorylation-regulated kinase 1A
• Protein Family: Dual specificity tyrosine-phosphorylation-regulated kinase
• UniProt Gene Name: DYRK1A
• UniProt Synonym Gene Names: DYRK; MNB; MNBH; MNBH; hMNB
• UniProt Entry Name: DYR1A_HUMAN

NCBI Description

This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

DYRK1A: a dual-specificity protein kinase of the DYRK family. Contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. May play a significant role in a pathway regulating cell proliferation and may be involved in brain development. Its gene is localized in the Down syndrome (DS) critical region of chromosome 21, and is a strong candidate gene for learning defects associated with Down syndrome. Overexpression in mice gives rise to neurological abnormalities similar to those observed in DS. Five splice-variant isoforms have been described.

Protein type: Protein kinase, CMGC; EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Kinase, protein; CMGC group; DYRK family; Dyrk1 subfamily

Chromosomal Location of Human Ortholog: 21q22.13

Cellular Component: nucleoplasm; nuclear speck; ribonucleoprotein complex; nucleus

Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; protein self-association; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; tau protein binding; ATP binding; protein kinase activity

Biological Process: circadian rhythm; nervous system development; negative regulation of DNA damage response, signal transduction by p53 class mediator; peptidyl-serine phosphorylation; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; mitotic cell cycle; protein amino acid phosphorylation; regulation of alternative nuclear mRNA splicing, via spliceosome

Disease: Mental Retardation, Autosomal Dominant 7