Mouse Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) ELISA Kit | CFTR elisa kit

Mouse Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) ELISA Kit

Cat. Num. AAA2704442

• Gene Names: CFTR; CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1
• Reactivity: Mouse
• Synonyms: Cystic Fibrosis Transmembrane Conductance Regulator (CFTR); Mouse Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) ELISA Kit; ABC35; ABCC7; CF; CFTR/MRP; MRP7; TNR-CFTR; ATP-Binding Cassette Subfamily C; Member 7; Channel conductance-controlling ATPase; cAMP-dependent chloride channel; CFTR elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR).; No significant cross-reactivity or interference between Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) and analogues was observed.
• Sequence Length: 1480

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CFTR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystic Fibrosis Transmembrane Conductance Regulator (CFTR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cystic Fibrosis Transmembrane Conductance Regulator (CFTR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystic Fibrosis Transmembrane Conductance Regulator (CFTR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 90421313
• NCBI GeneID: 1080
• NCBI Accession #: NP_000483.3
• NCBI GenBank Nucleotide #: NM_000492.3
• UniProt Accession #: P13569; Q20BG8; Q20BH2; Q2I0A1; Q2I102
• Molecular Weight: 69,230 Da
• NCBI Official Full Name: cystic fibrosis transmembrane conductance regulator
• NCBI Official Synonym Full Names: cystic fibrosis transmembrane conductance regulator
• NCBI Official Symbol: CFTR
• NCBI Official Synonym Symbols: CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1
• NCBI Protein Information: cystic fibrosis transmembrane conductance regulator
• UniProt Protein Name: Cystic fibrosis transmembrane conductance regulator
• Protein Family: Cystic fibrosis transmembrane conductance regulator
• UniProt Gene Name: CFTR
• UniProt Synonym Gene Names: ABCC7; CFTR

NCBI Description

This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome. [provided by RefSeq, Aug 2017]

Uniprot Description

CFTR: a member of the MRP subfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. Functions as a chloride channel and controls the regulation of other transport pathways. Mutations have been observed in patients with the autosomal recessive disorders cystic fibrosis (CF) and congenital bilateral aplasia of the vas deferens (CBAVD). Alternative splice

Protein type: Channel, chloride; EC 3.6.3.49; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 7q31.2

Cellular Component: apical plasma membrane; cell surface; chloride channel complex; clathrin-coated vesicle membrane; cytoplasm; cytosol; early endosome; early endosome membrane; endoplasmic reticulum membrane; endosome membrane; extracellular exosome; integral component of membrane; integral component of plasma membrane; lysosomal membrane; plasma membrane; protein complex; recycling endosome; recycling endosome membrane

Molecular Function: anion transmembrane-transporting ATPase activity; ATP binding; ATPase activity; ATPase activity, coupled to transmembrane movement of substances; bicarbonate transmembrane transporter activity; channel-conductance-controlling ATPase activity; chloride channel activity; chloride channel inhibitor activity; chloride channel regulator activity; chloride transmembrane transporter activity; enzyme binding; PDZ domain binding; protein binding

Biological Process: bicarbonate transport; cellular response to cAMP; cellular response to forskolin; cholesterol biosynthetic process; cholesterol transport; intracellular pH elevation; membrane hyperpolarization; multicellular organismal water homeostasis; positive regulation of cyclic nucleotide-gated ion channel activity; positive regulation of exocytosis; protein deubiquitination; sperm capacitation; transmembrane transport; vesicle docking involved in exocytosis

Disease: Bronchiectasis With Or Without Elevated Sweat Chloride 1; Cystic Fibrosis; Pancreatitis, Hereditary; Vas Deferens, Congenital Bilateral Aplasia Of