Rabbit anti-Human Dynein IC2 Polyclonal Antibody | anti-IC2 antibody

Dynein IC2, axonemal (N105) Polyclonal Antibody

Cat. Num. AAA3013970

• Gene Names: DNAI2; DIC2; CILD9
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Dynein IC2; Polyclonal Antibody; axonemal (N105) Polyclonal Antibody; Dynein intermediate chain 2; axonemal; Axonemal dynein intermediate chain 2; DNAI2; anti-IC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Dynein IC2, axonemal (N105) polyclonal antibody detects endogenous levels of Dynein IC2, axonemal protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 593

• Applicable Applications for anti-IC2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 75-135 of Human Dynein IC2, axonemal.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Dynein IC2, axonemal (N105) polyclonal antibody at 1:500 dilutionLane1:A2780 whole cell lysate (40ug) Lane2:Beas-2B whole cell lysate (40ug) Lane3:SK-OVCAR3 whole cell lysate (40ug))

Related Product Information for anti-IC2 antibody

Dyneins are multi-subunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. Cytoplasmic or axonemal Dynein heavy, intermediate, light and light-intermediate chains are all components of minus end-directed motors; the complex transports cellular cargos towards the central region of the cell. Axonemal Dynein motors contain one to three nonidentical heavy chains and cause a sliding of microtubules in the axonemes of cilia and flagella in a mechanism necessary for cilia to beat and propel the cell. Cytoplasmic Dynein is an approximately 12 subunit complex of two heavy chains, 2 intermediate chains to anchor Dynein to its cargo, 4 smaller intermediate chains and several light chains. It performs functions necessary for cell survival such as organelle transport and centrosome assembly. The carboxy-terminus of Dynein is important for microtubule-dependent motility and is highly conserved, while the amino-terminal regions are more variable. Several proteins regulate Dynein activity, including dynactin, LIS1 and nudel (NudE-like).

NCBI and Uniprot Product Information

• NCBI GI #: 289802997
• NCBI GeneID: 64446
• NCBI Accession #: NP_001166281.1
• NCBI GenBank Nucleotide #: NP_001166281.1
• UniProt Accession #: Q9GZS0
• Molecular Weight: ~ 73kDa
• NCBI Official Full Name: dynein intermediate chain 2, axonemal isoform 2
• NCBI Official Synonym Full Names: dynein axonemal intermediate chain 2
• NCBI Official Symbol: DNAI2
• NCBI Official Synonym Symbols: DIC2; CILD9
• NCBI Protein Information: dynein intermediate chain 2, axonemal
• UniProt Protein Name: Dynein intermediate chain 2, axonemal
• UniProt Gene Name: DNAI2
• UniProt Entry Name: DNAI2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the dynein intermediate chain family, and is part of the dynein complex of respiratory cilia and sperm flagella. Mutations in this gene are associated with primary ciliary dyskinesia type 9. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

DNAI2: Part of the dynein complex of respiratory cilia. Defects in DNAI2 are the cause of primary ciliary dyskinesia type 9 (CILD9). CILD is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. Belongs to the dynein intermediate chain family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis; Microtubule-binding

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: microtubule; axonemal dynein complex; axoneme

Molecular Function: protein binding; microtubule motor activity

Biological Process: metabolic process; cilium biogenesis

Disease: Ciliary Dyskinesia, Primary, 9