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Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissueAll lanes: DYM antibody at 3.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 76, 55 KDaObserved band size: 55 KDa)

Rabbit anti-Human, Mouse DYM Polyclonal Antibody | anti-DYM antibody

DYM Antibody

Gene Names
DYM; DMC; SMC
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>95%, Protein G purified
Synonyms
DYM; Polyclonal Antibody; DYM Antibody; Dymeclin; Dyggve-Melchior-Clausen syndrome protein; anti-DYM antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
669
Applicable Applications for anti-DYM antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:5000
IHC: 1:500-1:1000
Immunogen
Recombinant Human Dymeclin protein (224-348AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissueAll lanes: DYM antibody at 3.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 76, 55 KDaObserved band size: 55 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissueAll lanes: DYM antibody at 3.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 76, 55 KDaObserved band size: 55 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7108844 diluted at 1:500 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7108844 diluted at 1:500 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(IHC image of MBS7108844 diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7108844 diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Related Product Information for anti-DYM antibody
Necessary for correct organization of Golgi apparatus. Involved in bone development.
Product Categories/Family for anti-DYM antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,425 Da
NCBI Official Full Name
dymeclin isoform 1
NCBI Official Synonym Full Names
dymeclin
NCBI Official Symbol
DYM
NCBI Official Synonym Symbols
DMC; SMC
NCBI Protein Information
dymeclin
UniProt Protein Name
Dymeclin
Protein Family
UniProt Gene Name
DYM

NCBI Description

This gene encodes a protein which regulates Golgi-associated secretory pathways that are essential to endochondral bone formation during early development. This gene is also believed to play a role in early brain development. This gene is widely expressed in embryos and is particularly abundant in chodrocytes and brain tissues. It encodes a peripheral membrane protein which shuttles between the cytosol and Golgi complex. Mutations in this gene are associated with two types of recessive osteochondrodysplasia: Dyggve-Melchior-Clausen (DMC) dysplasia and Smith-McCort (SMC) dysplasia. [provided by RefSeq, Jun 2017]

Uniprot Description

Necessary for correct organization of Golgi apparatus. Involved in bone development.

Research Articles on DYM

Similar Products

Product Notes

The DYM dym (Catalog #AAA7108844) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DYM Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DYM can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:5000 IHC: 1:500-1:1000. Researchers should empirically determine the suitability of the DYM dym for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DYM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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