Rabbit anti-Human CA9 Polyclonal Antibody | anti-CA9 antibody

CA9, ID (Carbonic Anhydrase 9, Carbonic Anhydrase IX, Carbonate Dehydratase IX, CA-IX, CAIX, Membrane Antigen MN, P54/58N, Renal Cell Carcinoma-associated Antigen G250, RCC-associated Antigen G250, pMW1, G250, MN) (Biotin)

Cat. Num. AAA6467033

• Gene Names: CA9; MN; CAIX
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CA9; Polyclonal Antibody; ID (Carbonic Anhydrase 9; Carbonic Anhydrase IX; Carbonate Dehydratase IX; CA-IX; CAIX; Membrane Antigen MN; P54/58N; Renal Cell Carcinoma-associated Antigen G250; RCC-associated Antigen G250; pMW1; G250; MN) (Biotin); anti-CA9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CA9.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CA9 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CA9 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CA9 antibody (Center) in Hela cell line lysates (35ug/lane). CA9 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using CA9 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Immunofluorescence analysis of CA9 Antibody (Center) with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.)

Product Categories/Family for anti-CA9 antibody

Antibodies; Enzymes

References

1. Grabmaier, K., et al., Oncogene 23(33):5624-5631 (2004). 2. Kaluzova, M., et al., Mol. Cell. Biol. 24(13):5757-5766 (2004). 3. Span, P.N., et al., Br. J. Cancer 89(2):271-276 (2003). 4. Hedley, D., et al., Clin. Cancer Res. 9(15):5666-5674 (2003). 5. Bui, M.H., et al., Clin. Cancer Res. 9(2):802-811 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 169636420
• NCBI GeneID: 768
• NCBI Accession #: NP_001207
• NCBI GenBank Nucleotide #: NM_001216.2
• UniProt Accession #: Q16790; Q5T4R1
• Molecular Weight: 49,698 Da
• NCBI Official Full Name: carbonic anhydrase 9
• NCBI Official Synonym Full Names: carbonic anhydrase IX
• NCBI Official Symbol: CA9
• NCBI Official Synonym Symbols: MN; CAIX
• NCBI Protein Information: carbonic anhydrase 9
• UniProt Protein Name: Carbonic anhydrase 9
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA9
• UniProt Synonym Gene Names: G250; MN; CA-IX; CAIX; RCC-associated antigen G250

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

Uniprot Description

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.