Chicken anti-Human DUT / DUTPase Polyclonal Antibody | anti-DUT antibody

Chicken Polyclonal (IgY) to Human DUT / DUTPase

Cat. Num. AAA242267

• Gene Names: DUT; dUTPase
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: DUT / DUTPase; Polyclonal Antibody; Chicken Polyclonal (IgY) to Human DUT / DUTPase; Anti-DUT / DUTPase Antibody (aa1-52) IHC-plus; DUT; Deoxyuridine triphosphatase; DUTP pyrophosphatase; DUT-N; DUTP nucleotidohydrolase; DUTPase; Human DUT; anti-DUT antibody

• Host: Chicken
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Amino acids 1-52 of human DUT
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS. No preservative added.
• Sequence Length: 164

• Applicable Applications for anti-DUT antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Recombinant human DUT.
• Immunogen Type: Recombinant protein
• Immunogen: DUT / DUTPase antibody was raised against recombinant human DUT.
• Antigen Modification: aa1-52
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-DUT antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-DUT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 4503423
• NCBI GeneID: 1854
• NCBI Accession #: NP_001939.1
• NCBI GenBank Nucleotide #: NM_001948.3
• UniProt Accession #: P33316; O14785; Q16708; Q16860; Q6FHN1; Q6NSA3; Q96Q81; A8K650; B4DPR5
• Molecular Weight: 17,748 Da
• NCBI Official Full Name: deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: deoxyuridine triphosphatase
• NCBI Official Symbol: DUT
• NCBI Official Synonym Symbols: dUTPase
• NCBI Protein Information: deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; dUTP pyrophosphatase; dUTP nucleotidohydrolase
• UniProt Protein Name: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
• Protein Family: Deoxyuridine 5'-triphosphate nucleotidohydrolase
• UniProt Gene Name: DUT
• UniProt Synonym Gene Names: dUTPase
• UniProt Entry Name: DUT_HUMAN

NCBI Description

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008]

Uniprot Description

dUTPase: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. Belongs to the dUTPase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Nucleotide Metabolism - pyrimidine; Hydrolase; DNA replication; Motility/polarity/chemotaxis; Mitochondrial; EC 3.6.1.23

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: nucleoplasm; mitochondrion; nucleus

Molecular Function: protein binding; magnesium ion binding; dUTP diphosphatase activity

Biological Process: dUTP catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; DNA replication; dUMP biosynthetic process