Mouse anti-Human DUT / DUTPase Monoclonal Antibody | anti-DUT antibody

Mouse Monoclonal [clone 1C9] (IgG1,k) to Human DUT / DUTPase

Cat. Num. AAA246187

• Gene Names: DUT; dUTPase
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: DUT / DUTPase; Monoclonal Antibody; Mouse Monoclonal [clone 1C9] (IgG1; k) to Human DUT / DUTPase; Anti-DUT / DUTPase Antibody (clone 1C9) IHC-plus; DUT; Deoxyuridine triphosphatase; DUTP pyrophosphatase; DUT-N; DUTP nucleotidohydrolase; DUTPase; Human DUT; anti-DUT antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1C9
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Sequence Length: 164

• Applicable Applications for anti-DUT antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB; Usage: Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
• Target Species: Human
• Immunogen Description: DUT (AAH33645, 68 a.a. ~ 164 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: DUT / DUTPase antibody was raised against dUT (AAH33645, 68 a.a. ~ 164 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-DUT antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-DUT antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to DUT on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(Western blot of DUT expression in transfected 293T cell line by DUT monoclonal antibody clone 1C9.)

ELISA (EIA)

(Detection limit for recombinant GST tagged DUT is approximately 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4503423
• NCBI GeneID: 1854
• NCBI Accession #: NP_001939.1
• NCBI GenBank Nucleotide #: NM_001948.3
• UniProt Accession #: P33316; O14785; Q16708; Q16860; Q6FHN1; Q6NSA3; Q96Q81; A8K650; B4DPR5
• Molecular Weight: 17,748 Da
• NCBI Official Full Name: deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: deoxyuridine triphosphatase
• NCBI Official Symbol: DUT
• NCBI Official Synonym Symbols: dUTPase
• NCBI Protein Information: deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; dUTP pyrophosphatase; dUTP nucleotidohydrolase
• UniProt Protein Name: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
• Protein Family: Deoxyuridine 5'-triphosphate nucleotidohydrolase
• UniProt Gene Name: DUT
• UniProt Synonym Gene Names: dUTPase
• UniProt Entry Name: DUT_HUMAN

NCBI Description

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008]

Uniprot Description

dUTPase: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. Belongs to the dUTPase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA replication; Nucleotide Metabolism - pyrimidine; Hydrolase; Mitochondrial; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; EC 3.6.1.23

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: nucleoplasm; mitochondrion; nucleus

Molecular Function: protein binding; magnesium ion binding; dUTP diphosphatase activity

Biological Process: dUTP catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; pyrimidine nucleoside biosynthetic process; DNA replication; dUMP biosynthetic process