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Rabbit anti-Human, Mouse DUT Polyclonal Antibody | anti-DUT antibody

DUT Antibody

Gene Names
DUT; dUTPase
Reactivity
Human, Mouse
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
DUT; Polyclonal Antibody; DUT Antibody; Deoxyuridine 5' triphosphate nucleotidohydrolase; nuclear isoform; Deoxyuridine 5'-triphosphate nucleotidohydrolase; mitochondrial; Deoxyuridine triphosphatase; dut; DUT_HUMAN; dUTP nucleotidohydrolase; dUTP pyrophosphatase; dUTPase; FLJ20622; anti-DUT antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
DUT Antibody detects endogenous levels of DUT.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-DUT antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human DUT.
Conjugation
Unconjugated
Similarity
Belongs to the dUTPase family.
Post Translational Modifications
Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated.
Subunit Structure
Homotrimer.
Tissue Specificity
Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level).
Function
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Product Categories/Family for anti-DUT antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
252
NCBI Official Full Name
Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
NCBI Official Synonym Full Names
deoxyuridine triphosphatase
NCBI Official Symbol
DUT
NCBI Official Synonym Symbols
dUTPase
NCBI Protein Information
deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; dUTP pyrophosphatase; dUTP nucleotidohydrolase
UniProt Protein Name
Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
UniProt Gene Name
DUT
UniProt Synonym Gene Names
dUTPase
UniProt Entry Name
DUT_HUMAN

NCBI Description

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008]

Uniprot Description

dUTPase: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. Belongs to the dUTPase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Motility/polarity/chemotaxis; Mitochondrial; DNA replication; Nuclear receptor co-regulator; Nucleotide Metabolism - pyrimidine; EC 3.6.1.23

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: nucleoplasm; mitochondrion; nucleus

Molecular Function: protein binding; magnesium ion binding; dUTP diphosphatase activity

Biological Process: dUTP catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; DNA replication; dUMP biosynthetic process

Research Articles on DUT

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Product Notes

The DUT dut (Catalog #AAA9629905) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DUT Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DUT can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the DUT dut for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DUT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.
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