Rabbit anti-Human DUSP13-M1 Polyclonal Antibody | anti-DUSP13 antibody

DUSP13-M1 Antibody (N-term)

Cat. Num. AAA9215317

• Gene Names: DUSP13; BEDP; MDSP; TMDP; SKRP4; DUSP13A; DUSP13B
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: DUSP13-M1; Polyclonal Antibody; DUSP13-M1 Antibody (N-term); Dual specificity protein phosphatase 13 isoform B; DUSP13B; Dual specificity phosphatase SKRP4; Testis- and skeletal-muscle-specific DSP; DUSP13; TMDP; anti-DUSP13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This DUSP13-M1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DUSP13-M1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 1-30
• Sequence Length: 188

• Applicable Applications for anti-DUSP13 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of DUSP13 (arrow) using DUSP13-M1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DUSP13 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-DUSP13 antibody

Dual-specificity phosphatases, a subfamily of protein-tyrosine phosphatases, play important roles in signal transduction, cell cycle progression, and tumor suppression. The cDNA encoding a novel phosphatase, PIR1, phosphatase that interacts with RNA/RNP complex 1. Sequence analysis revealed that the predicted 329-amino acid protein has homology to several dual-specificity phosphatases and contains 2 stretches of arginine-rich sequence similar to those found in some RNA-binding proteins. In vitro, recombinant protein displays protein-tyrosine phosphatase activity and binds directly to RNA.

Product Categories/Family for anti-DUSP13 antibody

Cell Biology; Developmental Biology; Signal Transduction

References

Nakamura K., Shima H., Watanabe M., Haneji T., Kikuchi K.Biochem. J. 344:819-825(1999).
Deloukas et al. Nature 429:375-381(2004).
Strausberg et al. Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 56237018
• NCBI GeneID: 51207
• NCBI Accession #: NP_057448.3
• NCBI GenBank Nucleotide #: NM_016364.3
• UniProt Accession #: Q9UII6; Q5JSC6; Q6IAR0; Q96GC2; A8K776; A8K782; B3KPY1; B3KXT0; B4DUK0; Q6B8I1
• Molecular Weight: 22149
• NCBI Official Full Name: dual specificity protein phosphatase 13 isoform 6
• NCBI Official Synonym Full Names: dual specificity phosphatase 13
• NCBI Official Symbol: DUSP13
• NCBI Official Synonym Symbols: BEDP; MDSP; TMDP; SKRP4; DUSP13A; DUSP13B
• NCBI Protein Information: dual specificity protein phosphatase 13
• UniProt Protein Name: Dual specificity protein phosphatase 13 isoform B
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP13
• UniProt Synonym Gene Names: DUSP13B; TMDP; DUSP13B
• UniProt Entry Name: DS13B_HUMAN

NCBI Description

Members of the protein-tyrosine phosphatase superfamily cooperate with protein kinases to regulate cell proliferation and differentiation. This superfamily is separated into two families based on the substrate that is dephosphorylated. One family, the dual specificity phosphatases (DSPs) acts on both phosphotyrosine and phosphoserine/threonine residues. This gene encodes different but related DSP proteins through the use of non-overlapping open reading frames, alternate splicing, and presumed different transcription promoters. Expression of the distinct proteins from this gene has been found to be tissue specific and the proteins may be involved in postnatal development of specific tissues. A protein encoded by the upstream ORF was found in skeletal muscle, whereas the encoded protein from the downstream ORF was found only in testis. In mouse, a similar pattern of expression was found. Multiple alternatively spliced transcript variants were described, but the full-length sequence of only some were determined. [provided by RefSeq, Jul 2008]

Uniprot Description

DUSP13: May be involved in the regulation of meiosis and/or differentiation of testicular germ cells during spermatogenesis. Exhibits intrinsic phosphatase activity towards both phospho- seryl/threonyl and -tyrosyl residues of myelin basic protein, with similar specific activities in vitro. Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity; Cell cycle regulation; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: cytoplasm

Molecular Function: protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity

Biological Process: meiosis; spermatogenesis; protein amino acid dephosphorylation