Rabbit anti-Human DULLARD Polyclonal Antibody | anti-DULLARD antibody

DULLARD, ID (CTDNEP1, DULLARD, CTD nuclear envelope phosphatase 1, Serine/threonine-protein phosphatase dullard) (MaxLight 550)

Cat. Num. AAA6293704

• Gene Names: CTDNEP1; NET56; DULLARD; HSA011916
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DULLARD; Polyclonal Antibody; ID (CTDNEP1; CTD nuclear envelope phosphatase 1; Serine/threonine-protein phosphatase dullard) (MaxLight 550); CTDNEP1; Serine/threonine-protein phosphatase dullard; anti-DULLARD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-DULLARD antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DULLARD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-168 amino acids from the Central region of human DULLARD.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-DULLARD antibody

Serine/threonine phosphatase which may be required for proper nuclear membrane morphology. Involved in LPIN1 dephosphorylation. May antagonize BMP signaling.

Product Categories/Family for anti-DULLARD antibody

Antibodies; Enzymes; Phosphatase

References

Kim, Y., et al. Proc. Natl. Acad. Sci. U.S.A. 104(16):6596-6601(2007). Zhang, Y., et al. Mol. Cell 24(5):759-770(2006). Satow, R., et al. Dev. Cell 11(6):763-774(2006). Satow, R., et al. Biochem. Biophys. Res. Commun. 295(1):85-91(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 219555649
• NCBI GeneID: 23399
• NCBI Accession #: NP_001137247.1
• NCBI GenBank Nucleotide #: NM_001143775.1
• UniProt Accession #: O95476; Q96GQ9; D3DTN7
• Molecular Weight: 28,377 Da
• NCBI Official Full Name: CTD nuclear envelope phosphatase 1
• NCBI Official Synonym Full Names: CTD nuclear envelope phosphatase 1
• NCBI Official Symbol: CTDNEP1
• NCBI Official Synonym Symbols: NET56; DULLARD; HSA011916
• NCBI Protein Information: CTD nuclear envelope phosphatase 1
• UniProt Protein Name: CTD nuclear envelope phosphatase 1
• UniProt Gene Name: CTDNEP1
• UniProt Synonym Gene Names: DULLARD
• UniProt Entry Name: CNEP1_HUMAN

Uniprot Description

Function: Serine/threonine protein phosphatase forming with CNEP1R1 an active phosphatase complex that dephosphorylates and may activate LPIN1 and LPIN2. LPIN1 and LPIN2 are phosphatidate phosphatases that catalyze the conversion of phosphatidic acid to diacylglycerol and control the metabolism of fatty acids at differents levels. May indirectly modulate the lipid composition of nuclear and/or endoplasmic reticulum membranes and be required for proper nuclear membrane morphology and/or dynamics. May also indirectly regulate the production of lipid droplets and triacylglycerol. May antagonize BMP signaling. Ref.7 Ref.8

Catalytic activity: A phosphoprotein + H2O = a protein + phosphate.

Subunit structure: Interacts with CNEP1R1; the complex dephosphorylates LPIN1 and LPIN2. Ref.8

Subcellular location: Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein Ref.2 Ref.7 Ref.8.

Tissue specificity: Muscle specific with lower expression in other metabolic tissues. Ref.8

Sequence similarities: Belongs to the dullard family.Contains 1 FCP1 homology domain.

Biophysicochemical propertiesKinetic parameters:KM=18 mM for p-nitrophenylphosphate Ref.7pH dependence:Optimum pH is 5.5.