Rabbit anti-Human Dual specificity mitogen-activated protein kinase kinase 3 Polyclonal Antibody | anti-MAP2K3 antibody

Rabbit anti-human Dual specificity mitogen-activated protein kinase kinase 3 polyclonal Antibody

Cat. Num. AAA7005370

• Gene Names: MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Synonyms: Dual specificity mitogen-activated protein kinase kinase 3; Polyclonal Antibody; Rabbit anti-human Dual specificity mitogen-activated protein kinase kinase 3 polyclonal Antibody; MAPK/ERK kinase 3 MAP2K3 MEK3; MKK3; PRKMK3; SKK2; anti-MAP2K3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation purified
• Form/Format: Liquid
• Sequence Length: 318

• Applicable Applications for anti-MAP2K3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Recommended dilution: WB:1:500-2000
• Immunogen: Recombinant human Dual specificity mitogen-activated protein kinase kinase 3 protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-13069 / sc-961 / sc-959 / sc-960
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: MAP2K3 antibody at 10 ug/mlLane 1: Raw264.7 whole cell lysateLane 2: Mouse skeletal muscle tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 40,37 kDaObserved band size: 38,26 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded MBS7005370 using human skeletal muscle tissue at dilution 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS7005370 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF)

(Immunofluorescent analysis of A549 cells using MBS7005370 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-MAP2K3 antibody

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.

This protein is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.

Product Categories/Family for anti-MAP2K3 antibody

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 938403439
• NCBI GeneID: 5606
• NCBI Accession #: NP_001303261.1
• NCBI GenBank Nucleotide #: NM_001316332.1
• UniProt Accession #: P46734; Q99441; Q9UE71; Q9UE72; B3KSK7
• Molecular Weight: 39,940 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 3 isoform A
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 3
• NCBI Official Symbol: MAP2K3
• NCBI Official Synonym Symbols: MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 3
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 3
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K3
• UniProt Synonym Gene Names: MEK3; MKK3; PRKMK3; SKK2; MAP kinase kinase 3; MAPKK 3; MEK 3; SAPK kinase 2; SAPKK-2; SAPKK2
• UniProt Entry Name: MP2K3_HUMAN

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MKK3: a dual-specificity protein kinase of the STE7 family. Activates p38 MAP kinase by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by cytokines and environmental stress in vivo. A deletion and two point mutants found in colon cancer cell lines. Three splice-variant isoforms have been described.

Protein type: Protein kinase, STE; EC 2.7.12.2; Kinase, protein; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytoplasm; cytosol; membrane; nucleoplasm

Molecular Function: ATP binding; MAP kinase kinase activity; protein binding; protein kinase binding; protein serine/threonine kinase activity; protein-tyrosine kinase activity

Biological Process: activation of MAPK activity; cardiac muscle contraction; inflammatory response; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of protein kinase activity; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of cytokine biosynthetic process; regulation of mitotic cell cycle; signal transduction; stress-activated MAPK cascade; stress-activated protein kinase signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway