Rabbit anti-Human DTX3L Polyclonal Antibody | anti-DTX3L antibody

DTX3L Antibody

Cat. Num. AAA9631001

• Gene Names: DTX3L; BBAP
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: DTX3L; Polyclonal Antibody; DTX3L Antibody; B lymphoma and BAL associated protein; B-lymphoma- and BAL-associated protein; BBAP; Deltex 3 like; DTX 3L; DTX3L_HUMAN; E3 ubiquitin-protein ligase DTX3L; Protein deltex 3 like; Protein deltex 3 like protein; Protein deltex-3-like; Rhysin 2; Rhysin-2; Rhysin2; anti-DTX3L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: DTX3L Antibody detects endogenous levels of DTX3L.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-DTX3L antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human DTX3L.
• Conjugation: Unconjugated
• Similarity: Belongs to the Deltex family.
• Post Translational Modifications: Autoubiquitinated.
• Subunit Structure: Homodimer and heterodimer. Can heterodimerize with DTX1, enhancing its ubiquitin ligase activity in vitro. Interacts (via N-terminus) with ADP ribosyltransferase PARP9/BAL1 (via PARP catalytic domain) forming a stable complex; the interaction is required to activate PARP9 but is dispensable for DTX3L catalytic activity. Forms a complex with STAT1 and PARP9 independently of IFNB1 or IFNG-mediated STAT1 'Tyr-701' phosphorylation. Found in a complex with PARP9, STAT1 and HIST1H2BH. Found in a complex with E3 ligase ITCH and ESCRT-0 components HGS and STAM. Interacts (via C-terminus) with ITCH; the interaction is increased upon CXCL12 stimulation and inhibits ITCH catalytic activity; the interaction is direct. Interacts with HGS and STAM; the interaction brings together HGS and STAM and promotes their recruitment to early endosomes.(Microbial infection) Interacts with encephalomyocarditis virus (EMCV) C3 protease; the interaction results in C3 protease 'Lys-48'-linked ubiquitination.(Microbial infection) Interacts with human rhinovirus (HRV) C3 protease; the interaction results in C3 protease 'Lys-48'-linked ubiquitination.
• Function: E3 ubiquitin-protein ligase which, in association with ADP-ribosyltransferase PARP9, plays a role in DNA damage repair and in interferon-mediated antiviral responses. Monoubiquitinates several histones, including histone H2A, H2B, H3 and H4. In response to DNA damage, mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1). The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. By monoubiquitinating histone H2B HIST1H2BH/H2BJ and thereby promoting chromatin remodeling, positively regulates STAT1-dependent interferon-stimulated gene transcription and thus STAT1-mediated control of viral replication. Independently of its catalytic activity, promotes the sorting of chemokine receptor CXCR4 from early endosome to lysosome following CXCL12 stimulation by reducing E3 ligase ITCH activity and thus ITCH-mediated ubiquitination of endosomal sorting complex required for transport ESCRT-0 components HGS and STAM. In addition, required for the recruitment of HGS and STAM to early endosomes. In association with PARP9, plays a role in antiviral responses by mediating 'Lys-48'-linked ubiquitination of encephalomyocarditis virus (EMCV) and human rhinovirus (HRV) C3 proteases and thus promoting their proteosomal-mediated degradation.
• Note: Translocates to the nucleus in response to IFNG or IFNB1 stimulation (PubMed:26479788). Localizes at sites of DNA damage in a PARP1-dependent manner (PubMed:23230272). Localization to early endosomes is increased upon CXCL12 stimulation where it co-localizes with ITCH, CXCL4, HGS and STAM (PubMed:24790097). A minor proportion localizes to lysosomes (PubMed:24790097).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-DTX3L antibody

Cytoplasm; Nucleus; Early endosome membrane; Peripheral membrane protein; Cytoplasmic side; Lysosome membrane; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 19923717
• NCBI GeneID: 151636
• NCBI Accession #: NP_612144
• NCBI GenBank Nucleotide #: NM_138287
• UniProt Accession #: Q8TDB6; Q53ZZ3; Q5MJP7; B3KWH6
• Molecular Weight: 25,792 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase DTX3L
• NCBI Official Synonym Full Names: deltex 3 like, E3 ubiquitin ligase
• NCBI Official Symbol: DTX3L
• NCBI Official Synonym Symbols: BBAP
• NCBI Protein Information: E3 ubiquitin-protein ligase DTX3L; B-lymphoma- and BAL-associated protein; deltex 3-like; rhysin 2; rhysin-2; rhysin2
• UniProt Protein Name: E3 ubiquitin-protein ligase DTX3L
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: DTX3L
• UniProt Synonym Gene Names: BBAP; Rhysin2
• UniProt Entry Name: DTX3L_HUMAN