Western Blot (WB)
(WB Suggested Anti-DTL Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 721_B cell lysateDTL is supported by BioGPS gene expression data to be expressed in 721_B)
Rabbit DTL Polyclonal Antibody | anti-DTL antibody
DTL antibody - N-terminal region
Western Blot (WB)
(WB Suggested Anti-DTL Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 721_B cell lysateDTL is supported by BioGPS gene expression data to be expressed in 721_B)
Western Blot (WB)
(WB Suggested Anti-DTL Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 721_B cell lysateDTL is supported by BioGPS gene expression data to be expressed in 721_B)
Target Description: DTL is required for CDT1 proteolysis in response to DNA damage through the CUL4-DDB1 E3 ubiquitin-protein ligase. It seems to be necessary to ensure proper cell cycle regulation of DNA replication. DTL may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. It also may play a role in cell proliferation of NT2 embryonal carcinoma cells.
NCBI and Uniprot Product Information
Uniprot Description
RAMP: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis. Belongs to the WD repeat cdt2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin ligase; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: nucleoplasm; centrosome; nuclear membrane; intracellular membrane-bound organelle; cytoplasm; nucleolus; chromosome; nucleus
Molecular Function: protein binding; ubiquitin-protein ligase activity
Biological Process: protein monoubiquitination; ubiquitin-dependent protein catabolic process; bypass DNA synthesis; protein polyubiquitination; regulation of cell cycle; G2/M transition DNA damage checkpoint; DNA replication; response to DNA damage stimulus; response to UV
Research Articles on DTL
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Product Notes
The DTL dtl (Catalog #AAA3208133) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DTL antibody - N-terminal region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DTL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the DTL dtl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: VNQISGAHNT SDKQTPSKPK KKQNSKGLAP SVDFQQSVTV VLFQDENTLV. It is sometimes possible for the material contained within the vial of "DTL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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