Rabbit anti-Human DSG3 Polyclonal Antibody | anti-DSG3 antibody

DSG3, NT (DSG3, CDHF6, Desmoglein-3, 130kD pemphigus vulgaris antigen, Cadherin family member 6) (MaxLight 490)

Cat. Num. AAA6293593

• Gene Names: DSG3; PVA; CDHF6
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DSG3; Polyclonal Antibody; NT (DSG3; CDHF6; Desmoglein-3; 130kD pemphigus vulgaris antigen; Cadherin family member 6) (MaxLight 490); Cadherin family member 6; anti-DSG3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DSG3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-DSG3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 8-36 in the N-terminal region of human DSG3, UniProt Accession #NP_001935.2; P32926.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in WiDr cell line lysates (35ug/lane) usingMBS643558. This demonstrates the DSG3 antibody detected the DSG3 protein (arrow).)

Related Product Information for anti-DSG3 antibody

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.

Product Categories/Family for anti-DSG3 antibody

Antibodies; Cadherin

References

1. Baican, A., et al. Int. J. Dermatol. 49(7):768-774 (2010). 2. Jolly, P.S., et al. J. Biol. Chem. 285(12):8936-8941 (2010). 3. Capon, F., et al. Br. J. Dermatol. 161(6):1403-1405 (2009). 4. Aoyama, Y., et al. Exp. Dermatol. 18(4):404-408 (2009). 5. Latourelle, J.C., et al. BMC Med. Genet. 10, 98 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 119964718
• NCBI GeneID: 1830
• NCBI Accession #: NP_001935.2
• NCBI GenBank Nucleotide #: NM_001944.2
• UniProt Accession #: P32926; A8K2V2
• Molecular Weight: 107,533 Da
• NCBI Official Full Name: desmoglein-3 preproprotein
• NCBI Official Synonym Full Names: desmoglein 3
• NCBI Official Symbol: DSG3
• NCBI Official Synonym Symbols: PVA; CDHF6
• NCBI Protein Information: desmoglein-3; 130 kDa pemphigus vulgaris antigen; 130-kD pemphigus vulgaris antigen; cadherin family member 6
• UniProt Protein Name: Desmoglein-3
• Protein Family: Desmoglein
• UniProt Gene Name: DSG3
• UniProt Synonym Gene Names: CDHF6; PVA
• UniProt Entry Name: DSG3_HUMAN

Uniprot Description

DSG3: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 18q12.1

Cellular Component: desmosome; plasma membrane; integral to membrane; cytosol

Molecular Function: calcium ion binding

Biological Process: apoptosis; homophilic cell adhesion; cell structure disassembly during apoptosis