Western blot
(Western blot analysis of Phospho-DRP1 (Ser637) using 293 whole cell lysates)
Rabbit DRP1 (Phospho-Ser637) Polyclonal Antibody | anti-DRP1 antibody
Rabbit DRP1 (Phospho-Ser637) Antibody
IHC 1:50-1:200
IF 1:100-1:500
ELISA(peptide) 1:20000-1:40000
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Western blot
(Western blot analysis of Phospho-DRP1 (Ser637) using 293 whole cell lysates)
Western blot
(Western blot analysis of Phospho-DRP1 (Ser637) using 293 whole cell lysates)
Immunohistochemistry (IHC)
(At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/100 staining Human normal tissues adjacent to pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/100 staining Human normal tissues adjacent to pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/100 staining human liver carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(At 1/100 staining human liver carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunofluorescence (IF)
(Staining NCI-H1299 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG(H+L), diluted 1/600 was used as secondary antibody.)
Immunofluorescence (IF)
(Staining NCI-H1299 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG(H+L), diluted 1/600 was used as secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]
Uniprot Description
DRP1: Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis- dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport. Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Can self-assemble in multimeric ring-like structures. Interacts with BCL2L1; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Interacts with FIS1. Interacts with GSK3B and MARCH5. Interacts (via the GTPase and B domains) with UBE2I; the interaction promotes sumoylation of DNM1L, mainly in ite B domain. Interacts with PPP3CA; the interaction dephosphorylates DNM1L and regulates its transition to mitochondria. Interacts witn MID49 and MID51. Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons. Belongs to the dynamin family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial; Microtubule-binding; Motor; EC 3.6.5.5; Endoplasmic reticulum; Hydrolase; Apoptosis
Chromosomal Location of Human Ortholog: 12p11.21
Cellular Component: Golgi apparatus; microtubule; protein complex; intracellular membrane-bound organelle; mitochondrion; synaptic vesicle membrane; coated pit; cytosol; mitochondrial outer membrane; membrane; perinuclear region of cytoplasm; cytoplasm; peroxisome; cell junction
Molecular Function: GTPase activity; identical protein binding; protein binding; protein homodimerization activity; GTP binding; ubiquitin protein ligase binding; lipid binding
Biological Process: peroxisome fission; mitochondrial fission; release of cytochrome c from mitochondria; positive regulation of protein secretion; apoptosis; metabolic process; positive regulation of apoptosis; regulation of protein oligomerization; endocytosis; mitochondrial fragmentation during apoptosis; cell structure disassembly during apoptosis; protein homotetramerization
Disease: Encephalopathy, Lethal, Due To Defective Mitochondrial And Peroxisomal Fission
Research Articles on DRP1
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Product Notes
The DRP1 dnm1l (Catalog #AAA001660) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit DRP1 (Phospho-Ser637) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DRP1 (Phospho-Ser637) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Peptide ELISA. WB 1:1000-3000 IHC 1:50-1:200 IF 1:100-1:500 ELISA(peptide) 1:20000-1:40000 IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the DRP1 dnm1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DRP1 (Phospho-Ser637), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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