Mouse anti-Human IL1RAP/IL-1RAcP Monoclonal Antibody | anti-IL1RAP antibody

Anti-IL1RAP/IL-1RAcP Antibody (PE), Mouse Monoclonal

Cat. Num. AAA8101091

• Gene Names: IL1RAP; IL1R3; C3orf13; IL-1RAcP
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: IL1RAP/IL-1RAcP; Monoclonal Antibody; Anti-IL1RAP/IL-1RAcP Antibody (PE); Mouse Monoclonal; IL-1RAcP/IL-1R3 Antibody (PE); Mouse MAb; interleukin 1 receptor accessory protein; Anti-C3orf13 Antibody; Anti-IL-1RAcP Antibody; Anti-IL1R3 Antibody; anti-IL1RAP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: 05
• Specificity: Human IL-1RAcP/IL-1R3
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.03%ProClin300
• Concentration: 10ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-IL1RAP antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Human IL-1RAcP/IL-1R3 protein
• Conjugation: PE
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-1RAcP/IL-1R3 (rh IL-1RAcP/IL-1R3) and conjugated with PE under optimum conditions, the unreacted PE was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze!

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of anti-human IL1R3 on human whole blood monocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable monocytes.)

Related Product Information for anti-IL1RAP antibody

Interleukin-1 receptor accessory protein (IL-1RAcP) also known as Interleukin-1 receptor member 3 (IL-1R3) is a cytokine receptor that binds interleukin 1. The IL-1 receptor accessory protein (IL1RAP) is a transmembrane protein that interacts with IL-1R and is required for IL-1 signal transduction. Interleukin 1 induces the synthesis of the acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. IL-1RAcP/IL-1R3 is a necessary part of the interleukin 1 receptor complex which initiates signaling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. IL-1RAcP/IL-1R3 mediates interleukin-1-dependent activation of NF-kappa-B. Isoform 1 is part of the membrane-bound form of the IL-1 receptor. Signaling involves the formation of a ternary complex containing IL1R1, TOLLIP, MYD88, and IRAK1 or IRAK2. Isoform 2 modulates the response to interleukins by associating with soluble IL1R1 and enhancing interleukin-binding to the decoy receptor.

Product Categories/Family for anti-IL1RAP antibody

Neuroinflammation; IL1 signaling pathway

References

Goldbach-Mansky R, et al. (2009) Autoinflammation: the prominent role of IL-1 in monogenic autoinflammatory diseases and implications for common illnesses. J Allergy Clin Immunol. 124(6): 1141-9.Johnston A, et al. (2011) IL-1F5, -F6, -F8, and -F9: a novel IL-1 family signaling system that is active in psoriasis and promotes keratinocyte antimicrobial peptide expression. J Immunol. 186(4): 2613-22.Ozaki K, et al. (2001) Effect of tumor weight and tube feeding on TNF-alpha and IL-1beta mRNA expression in the brain of mice. JPEN J Parenter Enteral Nutr. 25(6): 317-22.

NCBI and Uniprot Product Information

• NCBI GI #: 4504661
• NCBI GeneID: 3556
• NCBI Accession #: NP_002173.1
• NCBI GenBank Nucleotide #: NM_002182.3
• UniProt Accession #: Q9NPH3; O14915; Q86WJ7; B1NLD0; D3DNW0
• Molecular Weight: 65,418 Da
• NCBI Official Full Name: interleukin-1 receptor accessory protein isoform 1
• NCBI Official Synonym Full Names: interleukin 1 receptor accessory protein
• NCBI Official Symbol: IL1RAP
• NCBI Official Synonym Symbols: IL1R3; C3orf13; IL-1RAcP
• NCBI Protein Information: interleukin-1 receptor accessory protein; IL-1R3; interleukin-1 receptor 3; IL-1 receptor accessory protein; interleukin-1 receptor accessory protein beta
• UniProt Protein Name: Interleukin-1 receptor accessory protein
• Protein Family: Interleukin-1 receptor accessory protein
• UniProt Gene Name: IL1RAP
• UniProt Synonym Gene Names: C3orf13; IL1R3; IL-1 receptor accessory protein; IL-1RAcP; IL-1R-3; IL-1R3
• UniProt Entry Name: IL1AP_HUMAN

NCBI Description

Interleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. This gene encodes the interleukin 1 receptor accessory protein. The protein is a necessary part of the interleukin 1 receptor complex which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. [provided by RefSeq, Nov 2009]

Uniprot Description

IL1RAP: Coreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Belongs to the interleukin-1 receptor family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: membrane; integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: signal transducer activity; interleukin-1 receptor activity

Biological Process: interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; innate immune response; immune response; protein complex assembly; inflammatory response