Western Blot (WB)
(Western Blot analysis of Drosha on Hela cell and SiHa cell lysates using anti-Drosha antibody at 1/500 dilution.)
Rabbit anti-Human Drosha Polyclonal Antibody | anti-Drosha antibody
Drosha Polyclonal Antibody
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Drosha; Polyclonal Antibody; Drosha Polyclonal Antibody; RNase III; DROSHA; RN3; RNASE3L; RNASEN; Drosha double stranded RNA specific endoribonuclease; Drosha ribonuclease type III; Etohi2; HSA242976; Nuclear RNase III Drosha; p241; Protein Drosha; Putative protein p241 which interacts with transcription factor Sp1; Putative ribonuclease III; RANSE3L; Ribonuclease 3; Ribonuclease III; Ribonuclease III nuclear; Ribonuclease type III nuclear; RibonucleaseIII; RN 3; RNase 3; RNase3; RNaseIII; RNC; anti-Drosha antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Drosha polyclonal antibody detects endogenous levels of Drosha protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-Drosha antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
ICC: 1:50-1:200
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Drosha on Hela cell and SiHa cell lysates using anti-Drosha antibody at 1/500 dilution.)
Western Blot (WB)
(Western Blot analysis of Drosha on Hela cell and SiHa cell lysates using anti-Drosha antibody at 1/500 dilution.)
Immunocytochemistry (ICC)
(ICC staining Drosha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Drosha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-Drosha antibody
The ribonuclease III superfamily represents a structurally distinct group of double-strand-specific endonucleases with essential roles in RNA maturation, RNA decay, and gene silencing. Initial cleavage of microRNAs is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcripts (pri-miRNAs) in the nucleus. Human Drosha is a component of two multi-protein complexes. The larger complex contains multiple classes of RNA-associated proteins including RNA helicases, proteins that bind double-stranded RNA, novel heterogeneous nuclear ribonucleoproteins and the Ewing's sarcoma family of proteins. The smaller complex is composed of Drosha and the double-stranded-RNA-binding protein, DGCR8.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
159kDa
Similar Products
Product Notes
The Drosha (Catalog #AAA3012612) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Drosha Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Drosha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1,000 ICC: 1:50-1:200 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the Drosha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Drosha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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