Rabbit DROSHA Polyclonal Antibody | anti-DROSHA antibody

DROSHA Polyclonal Antibody

Cat. Num. AAA3007332

• Gene Names: DROSHA; RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DROSHA; Polyclonal Antibody; DROSHA Polyclonal Antibody; Ribonuclease 3 (EC: 3.1.26.3); Protein Drosha; Ribonuclease III; RNase III; p241; RN3; RNASE3L; RNASEN; anti-DROSHA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DROSHA polyclonal antibody detects endogenous levels of DROSHA protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1337

• Applicable Applications for anti-DROSHA antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human DROSHA.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DROSHA polyclonal antibody at 1:500 dilution Lane1:HEPG2 whole cell lysateLane2:The Brian tissue lysate of RatLane3:The Brian tissue lysate of Mouse)

Related Product Information for anti-DROSHA antibody

The ribonuclease III superfamily represents a structurally distinct group of double-strand-specific endonucleases with essential roles in RNA maturation, RNA decay, and gene silencing. Initial cleavage of microRNAs is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcripts (pri-miRNAs) in the nucleus. Human Drosha is a component of two multi-protein complexes. The larger complex contains multiple classes of RNA-associated proteins including RNA helicases, proteins that bind double-stranded RNA, novel heterogeneous nuclear ribonucleoproteins and the Ewing's sarcoma family of proteins. The smaller complex is composed of Drosha and the double-stranded-RNA-binding protein, DGCR8.

NCBI and Uniprot Product Information

• NCBI GI #: 155030236
• NCBI GeneID: 29102
• NCBI Accession #: NP_001093882.1
• NCBI GenBank Nucleotide #: NP_001093882.1
• UniProt Accession #: Q9NRR4
• Molecular Weight: ~ 159kDa
• NCBI Official Full Name: ribonuclease 3 isoform 2
• NCBI Official Synonym Full Names: drosha ribonuclease III
• NCBI Official Symbol: DROSHA
• NCBI Official Synonym Symbols: RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976
• NCBI Protein Information: ribonuclease 3
• UniProt Protein Name: Ribonuclease 3
• UniProt Gene Name: DROSHA
• UniProt Synonym Gene Names: RN3; RNASE3L; RNASEN; RNase III

NCBI Description

This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]

Uniprot Description

Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.