Guinea pig Drebrin Polyclonal Antibody | anti-DBN1 antibody

Drebrin

Cat. Num. AAA622428

• Gene Names: DBN1; D0S117E; DKFZp434D064
• Reactivity: Bovine, Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Purity: Serum; Whole antiserum.
• Synonyms: Drebrin; Polyclonal Antibody; Anti -Drebrin; anti-DBN1 antibody

• Host: Guinea pig
• Reactivity: Bovine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human drebrin isoforms E2 and A, widespread actin-associating proteins of 70kD (SDS-PAGE mobility shows Mr 120kD). Species Crossreactivity: bovine, rat, mouse.
• Purity/Purification: Serum; Whole antiserum.
• Form/Format: Supplied as a liquid in 0.09% sodium azide.

• Applicable Applications for anti-DBN1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence microscopy and Immunohistochemistry (frozen paraformaldehyde-fixed and paraffin embedded tissue, treatment recommended).,; Dilution: Immunohistochemistry: 1:100 1 hour at RT, longer for paraffin. Requires microwave antigen retrieval.; Optimal dilution to be determined by researcher.
• Immunogen: Synthetic human peptide (aa 324-343) (KLH).
• Positive Control: PLC, U333, HUVEC, HaCat, HeLa, MCF-7, SV-80, B1; B16 (mouse melanoma cells).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-DBN1 antibody

Drebrin has originally been described only in neuronal cells, but has been recently found also in the actin-rich lamellipodia and filopodia of motile cells. It is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells. Positive staining was found in many tissues, including diverse epithelia and carcinoma (e.g. in epidermis basal cells are positive, whereas overlying cells are negative; also positive: regenerating epithelium during wound healing; basal cell carcinoma; skin melanoma), specific types of endothelia and smooth muscle; especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis. Also positive: epithelia of hair follicles and eccrine sweat glands. Consistently negative were, however, hepatocytes and cross-striated muscle. Reactivities on Cultured PLC, U333, HUVEC, HaCat, HeLa, MCF-7, SV-80.

Product Categories/Family for anti-DBN1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 18426915
• NCBI GeneID: 1627
• NCBI Accession #: NP_004386.2
• NCBI GenBank Nucleotide #: NM_004395.3
• UniProt Accession #: Q16643; Q9UFZ5; B2RBG0
• Molecular Weight: 71,429 Da
• NCBI Official Full Name: drebrin isoform a
• NCBI Official Synonym Full Names: drebrin 1
• NCBI Official Symbol: DBN1
• NCBI Official Synonym Symbols: D0S117E; DKFZp434D064
• NCBI Protein Information: drebrin; drebrin E; drebrin-1; drebrin E2; OTTHUMP00000161448; OTTHUMP00000161449; OTTHUMP00000165798; OTTHUMP00000223806; developmentally-regulated brain protein
• UniProt Protein Name: Drebrin
• Protein Family: Drebrin
• UniProt Gene Name: DBN1
• UniProt Synonym Gene Names: D0S117E
• UniProt Entry Name: DREB_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. At least two alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq]

Uniprot Description

DBN1: a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. Expressed in brain neurons, heart, placenta, skeletal muscle, kidney and pancreas. Two alternatively spliced isoforms have been described.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: cytoplasm; dendrite; plasma membrane; gap junction; actomyosin; cell cortex; actin cytoskeleton

Molecular Function: protein binding; actin binding; profilin binding

Biological Process: maintenance of cellular protein localization; regulation of dendrite development; actin filament organization; regulation of neuronal synaptic plasticity