Rabbit anti-Human, Mouse DRD4 Polyclonal Antibody | anti-DRD4 antibody

DRD4, ID (D(2C) Dopamine Receptor, Dopamine D4 Receptor, D(4) Dopamine Receptor) (FITC)

Cat. Num. AAA6472914

• Gene Names: DRD4; D4DR
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: DRD4; Polyclonal Antibody; ID (D(2C) Dopamine Receptor; Dopamine D4 Receptor; D(4) Dopamine Receptor) (FITC); anti-DRD4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DRD4. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DRD4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human DRD4 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DRD4 Antibody (Center) in mouse heart tissue lysates (35ug/lane). DRD4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with DRD4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DRD4 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-DRD4 antibody

Antibodies; Receptors

References

1. Livingstone, C.D., et.al., Biochem. J. 287(PT 1), 277-282 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 32483397
• NCBI GeneID: 1815
• NCBI Accession #: NP_000788.2
• NCBI GenBank Nucleotide #: NM_000797.3
• UniProt Accession #: P21917; Q7Z7Q5; Q8NGM5; B0M0J7
• Molecular Weight: 48,361 Da
• NCBI Official Full Name: D(4) dopamine receptor
• NCBI Official Synonym Full Names: dopamine receptor D4
• NCBI Official Symbol: DRD4
• NCBI Official Synonym Symbols: D4DR
• NCBI Protein Information: D(4) dopamine receptor; D(4) dopamine receptor; dopamine D4 receptor; D(2C) dopamine receptor; seven transmembrane helix receptor
• UniProt Protein Name: D(4) dopamine receptor
• Protein Family: D(4) dopamine receptor
• UniProt Gene Name: DRD4
• UniProt Entry Name: DRD4_HUMAN

NCBI Description

This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats; the sequence shown contains four repeats. [provided by RefSeq, Jul 2008]

Uniprot Description

DRD4: Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Its activity is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: membrane; cell soma; integral to plasma membrane; plasma membrane; dendritic spine; terminal button; vesicle membrane; cell cortex

Molecular Function: dopamine D2 receptor-like receptor activity; identical protein binding; protein binding; potassium channel regulator activity; dopamine receptor activity; dopamine binding; drug binding; SH3 domain binding

Biological Process: circadian rhythm; synaptic transmission, dopaminergic; fear response; behavioral fear response; positive regulation of dopamine uptake; adult locomotory behavior; short-term memory; activation of MAPK activity; regulation of neurotransmitter secretion; positive regulation of kinase activity; regulation of calcium-mediated signaling; behavioral response to ethanol; dopamine metabolic process; dopamine receptor, adenylate cyclase inhibiting pathway; regulation of inhibitory postsynaptic membrane potential; negative regulation of cAMP biosynthetic process; behavioral response to cocaine; negative regulation of protein secretion; negative regulation of adenylate cyclase activity; regulation of dopamine metabolic process; response to amphetamine; social behavior; regulation of circadian rhythm; arachidonic acid secretion; cellular calcium ion homeostasis; olfactory learning; response to steroid hormone stimulus; retina development in camera-type eye; photoperiodism; dopamine receptor signaling pathway; positive regulation of sodium:hydrogen antiporter activity

Disease: Novelty Seeking Personality Trait; Attention Deficit-hyperactivity Disorder