Immunohistochemistry (IHC)
(MBS9609256 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human DRD1IP Polyclonal Antibody | anti-DRD1IP antibody
DRD1IP Antibody
Gene Names
CALY; NSG3; DRD1IP
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DRD1IP; Polyclonal Antibody; DRD1IP Antibody; CALCYON; Caly; CALY_HUMAN; D1 dopamine receptor interacting protein; Drd1ip; Neuron specific vesicular protein calcyon; Neuron-specific vesicular protein calcyon; NSG3; RP11-122K13.5; anti-DRD1IP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
DRD1IP antibody detects endogenous levels of total DRD1IP
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
217
Applicable Applications for anti-DRD1IP antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human DRD1IP
Subcellular Location
Cytoplasmic Vesicle Membrane. Cell Membrane.
Tissue Specificity
Expressed in the pyramidal cells of the prefrontal cortex, in hippothalamus and in caudate nucleus. No expression in spleen. Up-regulated in the prefrontal cortex of schizophrenic patients with nearly twice the levels of non-schizophrenics.
Predicted Cross Reactivity
Rabbit, Dog
Similarity
Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9609256 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609256 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of DRD1IP using RAW264.7 whole cell lysates)
Western Blot (WB)
(Western blot analysis of DRD1IP using RAW264.7 whole cell lysates)
Related Product Information for anti-DRD1IP antibody
Function: Interacts with clathrin light chain A and stimulates clathrin self-assembly and clathrin-mediated endocytosis.
Subunit Structure: Interacts with CLTA.
Post-translational Modifications: Glycosylated.
Similarity: Belongs to the NSG family.
Subunit Structure: Interacts with CLTA.
Post-translational Modifications: Glycosylated.
Similarity: Belongs to the NSG family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 23 kDa
Predicted: 24 kDa
Predicted: 24 kDa
NCBI Official Full Name
neuron-specific vesicular protein calcyon isoform 1
NCBI Official Synonym Full Names
calcyon neuron specific vesicular protein
NCBI Official Symbol
CALY
NCBI Official Synonym Symbols
NSG3; DRD1IP
NCBI Protein Information
neuron-specific vesicular protein calcyon
UniProt Protein Name
Neuron-specific vesicular protein calcyon
UniProt Gene Name
CALY
UniProt Synonym Gene Names
DRD1IP
NCBI Description
The protein encoded by this gene is a type II single transmembrane protein. It is required for maximal stimulated calcium release after stimulation of purinergic or muscarinic but not beta-adrenergic receptors. The encoded protein interacts with D1 dopamine receptor and may interact with other DA receptor subtypes and/or GPCRs. [provided by RefSeq, Jul 2008]
Uniprot Description
Interacts with clathrin light chain A and stimulates clathrin self-assembly and clathrin-mediated endocytosis.
Research Articles on DRD1IP
Similar Products
Product Notes
The DRD1IP caly (Catalog #AAA9609256) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DRD1IP Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DRD1IP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the DRD1IP caly for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DRD1IP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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