Western Blot (WB)
(Anti-DPYD antibody, MBS175344, Western blottingWB: MM231 Cell Lysate )
Rabbit DPYD Polyclonal Antibody | anti-DPYD antibody
Anti-DPYD antibody
Western Blot (WB)
(Anti-DPYD antibody, MBS175344, Western blottingWB: MM231 Cell Lysate )
Western Blot (WB)
(Anti-DPYD antibody, MBS175344, Western blottingWB: MM231 Cell Lysate )
Background: DPYD(Dihydropyrimidine Dehydrogenase), also called DPD, is an enzyme that in humans is encoded by the DPYD gene. The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. The structure of the DPYD gene contains 23 exons spanning about 950 kb. Using somatic cell hybrid strategies, the DPYD gene is mapped to the centromeric region of chromosome 1 between 1p22 and 1q21. By fluorescence in situ hybridization, the DPYD gene is mapped to 1p22. The highest level of DPD was found in monocytes followed by that in lymphocytes, granulocytes, and platelets, whereas no significant activity of DPD could be detected in erythrocytes. The activity of DPD in peripheral blood mononuclear cells was intermediate between that observed in monocytes and lymphocytes. By cDNA microarray, Western blot analysis, and luciferase reporter assay, the transcription factor LSF was identified as a positive regulator of DPYD.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Uniprot Description
DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family.
Protein type: Oxidoreductase; Xenobiotic Metabolism - drug metabolism - other enzymes; Other Amino Acids Metabolism - beta-alanine; Nucleotide Metabolism - pyrimidine; EC 1.3.1.2; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis
Chromosomal Location of Human Ortholog: 1p22
Cellular Component: cytoplasm; cytosol
Molecular Function: protein homodimerization activity; FAD binding; metal ion binding; dihydroorotate dehydrogenase activity; 4 iron, 4 sulfur cluster binding; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding
Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; UMP biosynthetic process; uracil catabolic process; beta-alanine biosynthetic process; thymine catabolic process; purine base catabolic process; thymidine catabolic process; pyrimidine base catabolic process
Disease: Dihydropyrimidine Dehydrogenase Deficiency
Research Articles on DPYD
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Product Notes
The DPYD dpyd (Catalog #AAA175344) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DPYD antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DPYD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the DPYD dpyd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DPYD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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