Rabbit anti-Human DPP8 Polyclonal Antibody | anti-DPP8 antibody

DPP8, NT (DPP8, DPRP1, Dipeptidyl peptidase 8, Dipeptidyl peptidase IV-related protein 1, Dipeptidyl peptidase VIII, Prolyl dipeptidase DPP8)

Cat. Num. AAA646865

• Gene Names: DPP8; DP8; DPRP1; MSTP141
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: DPP8; Polyclonal Antibody; NT (DPP8; DPRP1; Dipeptidyl peptidase 8; Dipeptidyl peptidase IV-related protein 1; Dipeptidyl peptidase VIII; Prolyl dipeptidase DPP8); Anti -DPP8; anti-DPP8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-DPP8 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:50-100; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: DPP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30~59 amino acids from the N-terminal region of human DPP8.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DPP8 Antibody (N-term) in Hela cell line lysates (35ug/lane). DPP8 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with DPP8 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DPP8 Antibody (N-term) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DPP8 antibody

DPP8 is a member of the peptidase S9B family, a small family of dipeptidyl peptidases that are able to cleave peptide substrates at a prolyl bond. The protein shares similarity with dipeptidyl peptidase IV in that it is ubiquitously expressed, and hydrolyzes the same substrates. These similarities suggest that, like dipeptidyl peptidase IV, this protein may play a role in T-cell activation and immune function.

Product Categories/Family for anti-DPP8 antibody

Antibodies; Abs to Enzymes; Protease (Proteinase; Peptidase)

NCBI and Uniprot Product Information

• NCBI GI #: 37577091
• NCBI GeneID: 54878
• NCBI Accession #: NP_932065.1
• NCBI GenBank Nucleotide #: NM_197961.2
• UniProt Accession #: Q6V1X1; Q7Z4C8; Q7Z4D3; Q7Z4E1; Q8IWG7; Q8NEM5; Q96JX1; Q9HBM2; Q9HBM3; Q9HBM4; Q9HBM5; Q9NXF4
• Molecular Weight: 103,358 Da
• NCBI Official Full Name: dipeptidyl peptidase 8 isoform 4
• NCBI Official Synonym Full Names: dipeptidyl-peptidase 8
• NCBI Official Symbol: DPP8
• NCBI Official Synonym Symbols: DP8; DPRP1; MSTP141
• NCBI Protein Information: dipeptidyl peptidase 8; DPRP-1; DPP VIII; dipeptidylpeptidase 8; prolyl dipeptidase DPP8; dipeptidyl peptidase VIII; dipeptidyl peptidase IV-related protein 1; dipeptidyl peptidase IV-related protein-1
• UniProt Protein Name: Dipeptidyl peptidase 8
• Protein Family: Dipeptidyl peptidase
• UniProt Gene Name: DPP8
• UniProt Synonym Gene Names: DPRP1; DP8; DPRP-1; DPP VIII
• UniProt Entry Name: DPP8_HUMAN

NCBI Description

This gene encodes a member of the peptidase S9B family, a small family of dipeptidyl peptidases that are able to cleave peptide substrates at a prolyl bond. The encoded protein shares similarity with dipeptidyl peptidase IV in that it is ubiquitously expressed, and hydrolyzes the same substrates. These similarities suggest that, like dipeptidyl peptidase IV, this protein may play a role in T-cell activation and immune function. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. May play a role in T-cell activation and immune function. Ref.1

Catalytic activity: Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline. Ref.1 Ref.2 Ref.7

Enzyme regulation: Inhibited by zinc. Inhibited by the serine proteinase inhibitor 4-(2-aminoethyl)benzenesulphonyl fluoride (AEBSF), and by di-isopropylfuorophosphate. Specifically inhibited by isoindoline derivatives. Ref.1 Ref.2 Ref.9

Subcellular location: Cytoplasm Ref.1 Ref.2 Ref.7.

Tissue specificity: Ubiquitously expressed, with highest levels in testis, placenta, prostate, muscle and brain. Ref.1 Ref.2

Induction: In activated T-cells. Ref.1 Ref.2 Ref.9

Sequence similarities: Belongs to the peptidase S9B family. DPPIV subfamily.

Biophysicochemical propertiesKinetic parameters:KM=208 µM for Ala-Pro-AMC Ref.2 Ref.7 Ref.8KM=130 µM for Ala-Pro-AFCKM=120 µM for H-Ala-Pro-pNaKM=1420 µM for H-Ala-Ala-pNaKM=310 µM for H-Arg-Pro-pNaKM=2050 µM for H-Asp-Pro-pNaKM=480 µM for H-Gly-Pro-pNapH dependence:Optimum pH is 7.4-8.5. Little activity below pH 6.5.

Sequence caution: The sequence AAQ13623.1 differs from that shown. Reason: Frameshift at several positions. The sequence AAQ13650.1 differs from that shown. Reason: Frameshift at several positions. The sequence AAQ13657.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA91059.1 differs from that shown. Reason: Frameshift at position 486. The sequence BAB55395.1 differs from that shown. Reason: Erroneous initiation.