Western Blot (WB)
(Western blot analysis of extracts of Mouse brain? tissue sample,using Doublecortin Antibody.)
Rabbit Doublecortin Polyclonal Antibody | anti-DBCN antibody
Doublecortin Antibody
Predicted: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Predicted: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
ELISA (Peptide): 1:20000-1:40000
Western Blot (WB)
(Western blot analysis of extracts of Mouse brain? tissue sample,using Doublecortin Antibody.)
Western Blot (WB)
(Western blot analysis of extracts of Mouse brain? tissue sample,using Doublecortin Antibody.)
Post Translational Modifications: Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules (By similarity). Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21 (By similarity). Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells.
Subunit Structure: Interacts with tubulin (PubMed:27292316). Interacts with USP9X (PubMed:24607389).
NCBI and Uniprot Product Information
Predicted: 41 kDa
NCBI Description
This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]
Uniprot Description
Doublecortin: Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration. Interacts with tubulin. Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: Xq22.3-q23
Cellular Component: microtubule; microtubule associated complex; neuron projection; cytoskeleton; cytosol
Molecular Function: protein binding; microtubule binding; protein kinase binding
Biological Process: nervous system development; axon guidance; central nervous system development; axon extension; dendrite morphogenesis; neuron migration; central nervous system projection neuron axonogenesis; brain development
Disease: Lissencephaly, X-linked, 1
Research Articles on DBCN
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Product Notes
The DBCN dcx (Catalog #AAA9610915) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Doublecortin Antibody reacts with Human, Mouse, Rat Predicted: Bovine, Horse, Sheep, Rabbit, Dog, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Doublecortin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the DBCN dcx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Doublecortin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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