Rabbit anti-Human Gab2 Polyclonal Antibody | anti-GAB2 antibody

Gab2, Phosphorylated (Grb2-Associated Binder2) (Tyr452)

Cat. Num. AAA618385

• Gene Names: GAB2; KIAA0571
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Synonyms: Gab2; Polyclonal Antibody; Phosphorylated (Grb2-Associated Binder2) (Tyr452); Anti -Gab2; anti-GAB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of Tyr452 phosphorylated Gab2. This antibody weakly crossreacts with phosphorylated Gab1.
• Purity/Purification: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

• Applicable Applications for anti-GAB2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr452 of human Gab2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from Flt3 ligand treated or untreated BaF3 cells, using MBS618385.)

Related Product Information for anti-GAB2 antibody

GRB-associated binder (Gab) is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs), such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane, undergoes phosphorylation and functions as a multiprotein assembling center (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of tyrosine 472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of tyrosines 307, 373 and 407 modulates the association to PLCgamma (8). Phosphorylation of tyrosines 627 and 659 are required for the binding and activation of protein tyrosine phosphatase SHP2 (6,9). Gab2 is also phosphorylated by tyrosine kinases (10,11). Tyrosine 452 is a potential binding site of p85, the regulatory subunit of PI3 kinase. Tyrosine 614 is essential for SHP2 association (11). Furthermore, Akt phosphorylates Gab2 at serine 149 and inhibits Gab2 tyrosine phosphorylation, suggesting that Akt is engaged in negative feedback regulation of Gab2 signaling (12).

Product Categories/Family for anti-GAB2 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 18105042
• NCBI GeneID: 9846
• NCBI Accession #: NP_536739.1
• NCBI GenBank Nucleotide #: NM_080491.2
• UniProt Accession #: Q9UQC2; O60317; A2RRM2; A6NEW9; A7MD36
• Molecular Weight: 74,458 Da
• NCBI Official Full Name: GRB2-associated-binding protein 2 isoform a
• NCBI Official Synonym Full Names: GRB2-associated binding protein 2
• NCBI Official Symbol: GAB2
• NCBI Official Synonym Symbols: KIAA0571
• NCBI Protein Information: GRB2-associated-binding protein 2; pp100; OTTHUMP00000234012; OTTHUMP00000234013; Grb2-associated binder 2; growth factor receptor bound protein 2-associated protein 2
• UniProt Protein Name: GRB2-associated-binding protein 2
• Protein Family: GRB2-associated-binding protein
• UniProt Gene Name: GAB2
• UniProt Synonym Gene Names: KIAA0571
• UniProt Entry Name: GAB2_HUMAN

NCBI Description

This gene is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq]

Uniprot Description

GAB2: an adaptor protein of the GRB2-associated binding protein family. Plays important roles in regulating the growth, differentiation, and function of many hematopoietic cell types.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q14.1

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; transmembrane receptor protein tyrosine kinase adaptor protein activity

Biological Process: integrin-mediated signaling pathway; cell migration; phosphoinositide-mediated signaling; positive regulation of cell proliferation; innate immune response; positive regulation of mast cell degranulation; osteoclast differentiation; transmembrane receptor protein tyrosine kinase signaling pathway