Western Blot (WB)
(Western blot analysis of Dopamine Receptor D5 expression in mouse brain (A) whole cell lysates.)
Rabbit Dopamine Receptor D5 Polyclonal Antibody | anti-DRD5 antibody
Anti-Dopamine Receptor D5 Antibody
Western Blot (WB)
(Western blot analysis of Dopamine Receptor D5 expression in mouse brain (A) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Dopamine Receptor D5 expression in mouse brain (A) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Dopamine Receptor D5 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Dopamine Receptor D5 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2. [provided by RefSeq, Jul 2008]
Uniprot Description
DRD5: Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Defects in DRD5 are a cause of benign essential blepharospasm (BEB). BEB is a primary focal dystonia affecting the orbicularis oculi muscles. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. BEB usually begins in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. Patients have normal eyes. The visual disturbance is due solely to the forced closure of the eyelids. In severe cases, this can lead to functional blindness. Belongs to the G-protein coupled receptor 1 family.
Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1
Chromosomal Location of Human Ortholog: 4p16.1
Cellular Component: integral to plasma membrane; nonmotile primary cilium; plasma membrane
Molecular Function: dopamine binding; dopamine D1 receptor-like receptor activity; dopamine receptor activity
Biological Process: adenylate cyclase activation; cellular calcium ion homeostasis; dopamine receptor, adenylate cyclase activating pathway; dopamine receptor, phospholipase C activating pathway; G-protein signaling, adenylate cyclase activating pathway; learning; negative regulation of NAD(P)H oxidase activity; positive regulation of adenylate cyclase activity; synaptic transmission, dopaminergic
Disease: Attention Deficit-hyperactivity Disorder; Blepharospasm, Benign Essential
Research Articles on DRD5
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Product Notes
The DRD5 drd5 (Catalog #AAA8247359) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Dopamine Receptor D5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Dopamine Receptor D5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/IC: 1/50 - 1/200. Researchers should empirically determine the suitability of the DRD5 drd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dopamine Receptor D5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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