Rabbit anti-Human, Mouse DOK5 Polyclonal Antibody | anti-DOK5 antibody

DOK5, CT (Docking Protein 5, Downstream Of Tyrosine Kinase 5, Insulin Receptor Substrate 6, IRS-6, IRS6, C20orf180) (APC)

Cat. Num. AAA6472846

• Gene Names: DOK5; IRS6; IRS-6; C20orf180
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: DOK5; Polyclonal Antibody; CT (Docking Protein 5; Downstream Of Tyrosine Kinase 5; Insulin Receptor Substrate 6; IRS-6; IRS6; C20orf180) (APC); anti-DOK5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DOK5. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-DOK5 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human DOK5 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-DOK5 C-term Pab is used in Western blot to detect DOK5 in mouse liver tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of DOK5 (arrow) using rabbit polyclonal DOK5 Antibody (L291). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DOK5 gene.)

Product Categories/Family for anti-DOK5 antibody

Antibodies; Binding Proteins

References

1. Cai, D., et al., J. Biol. Chem. 278(28):25323-25330 (2003). 2. Favre, C., et al., Genes Immun. 4(1):40-45 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 29544726
• NCBI GeneID: 55816
• NCBI Accession #: NP_060901.2
• NCBI GenBank Nucleotide #: NM_018431.4
• UniProt Accession #: Q9P104; Q5T7Y0; Q5TE53; Q8TEW7; Q96H13; Q9BZ24; Q9NQF4; Q9Y411
• Molecular Weight: 22,824 Da
• NCBI Official Full Name: docking protein 5 isoform a
• NCBI Official Synonym Full Names: docking protein 5
• NCBI Official Symbol: DOK5
• NCBI Official Synonym Symbols: IRS6; IRS-6; C20orf180
• NCBI Protein Information: docking protein 5
• UniProt Protein Name: Docking protein 5
• Protein Family: Docking protein
• UniProt Gene Name: DOK5
• UniProt Synonym Gene Names: C20orf180
• UniProt Entry Name: DOK5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. Unlike other DOK family proteins, this protein does not interact with RASGAP. This protein is up-regulated in patients with systemic sclerosis and is associated with fibrosis induced by insulin-like growth factor binding protein 5. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jun 2014]