Western Blot (WB)
(DOK5 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the DOK5 antibody detected the DOK5 protein (arrow).)
Rabbit anti-Human DOK5 Polyclonal Antibody | anti-DOK5 antibody
DOK5, NT (DOK5, C20orf180, Docking protein 5, Downstream of tyrosine kinase 5, Insulin receptor substrate 6)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(DOK5 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the DOK5 antibody detected the DOK5 protein (arrow).)
Western Blot (WB)
(DOK5 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the DOK5 antibody detected the DOK5 protein (arrow).)
Western Blot (WB)
(Western blot analysis of DOK5 (arrow) using rabbit polyclonal DOK5 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DOK5 gene.)
Western Blot (WB)
(Western blot analysis of DOK5 (arrow) using rabbit polyclonal DOK5 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DOK5 gene.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. In contrast to other DOK family proteins, this protein does not interact with RASGAP. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation.
Subunit structure: Interacts with phosphorylated RET. In contrast to other DOK proteins, it does not interact with RASGAP
By similarity.
Tissue specificity: Highest expression in skeletal muscle, lower in brain, heart and kidney. Also detected in activated peripheral blood T-lymphocytes. Ref.2 Ref.3
Domain: PTB domain mediates receptor interaction
By similarity.
Post-translational modification: Phosphorylated on tyrosine residues in response to insulin, IGF1 and GDNF. Ref.3
Sequence similarities: Belongs to the DOK family. Type B subfamily.Contains 1 IRS-type PTB domain.Contains 1 PH domain.
Research Articles on DOK5
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Product Notes
The DOK5 dok5 (Catalog #AAA645928) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DOK5, NT (DOK5, C20orf180, Docking protein 5, Downstream of tyrosine kinase 5, Insulin receptor substrate 6) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DOK5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the DOK5 dok5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DOK5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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