Rabbit anti-Human DOK3 Polyclonal Antibody | anti-DOK3 antibody

DOK3, CT (DOK3, Docking protein 3, Downstream of tyrosine kinase 3) (PE)

Cat. Num. AAA6293190

• Gene Names: DOK3; DOKL
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DOK3; Polyclonal Antibody; CT (DOK3; Docking protein 3; Downstream of tyrosine kinase 3) (PE); Downstream of tyrosine kinase 3; anti-DOK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-DOK3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DOK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-329 amino acids from the C-terminal region of human DOK3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DOK3 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the DOK3 antibody detected the DOK3 protein (arrow).)

Immunohistochemistry (IHC)

(DOK3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DOK3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-DOK3 antibody

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function (By similarity).

Product Categories/Family for anti-DOK3 antibody

Antibodies; Protein Kinases

References

Berger, A.H., et al. Nat. Genet. 42(3):216-223(2010). Senis, Y.A., et al. J. Thromb. Haemost. 7(10):1718-1726(2009). Honma, M., et al. Genes Cells 11(2):143-151(2006). Robson, J.D., et al. Mol. Cell. Biol. 24(6):2332-2343(2004). Favre, C., et al. Genes Immun. 4(1):40-45(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 221554537
• NCBI GeneID: 79930
• NCBI Accession #: NP_079148.2
• NCBI GenBank Nucleotide #: NM_024872.2
• UniProt Accession #: Q7L591; Q8N864; Q9BQB3; Q9H666; E9PAT0; H7BXS0
• Molecular Weight: 24,737 Da
• NCBI Official Full Name: docking protein 3 isoform 1
• NCBI Official Synonym Full Names: docking protein 3
• NCBI Official Symbol: DOK3
• NCBI Official Synonym Symbols: DOKL
• NCBI Protein Information: docking protein 3; Dok-like protein; downstream of tyrosine kinase 3
• UniProt Protein Name: Docking protein 3
• Protein Family: Docking protein
• UniProt Gene Name: DOK3
• UniProt Entry Name: DOK3_HUMAN

Uniprot Description

DOK3: docking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with SHIP. May modulate Abl function. Upon tyrosine phosphorylation, Dok3 interacts with CSK and SHIP via their SH2 domains. Both pY381 and pY398 are required for interaction with SHIP. Only pY381 is required for interaction with CSK. Binds ABL through the PTB domain and in a kinase-dependent manner. On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Does not interact with RasGAP. Four isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: cytoplasm; plasma membrane

Molecular Function: protein binding; insulin receptor binding

Biological Process: Ras protein signal transduction