Rabbit Dok1 (Tyr-362) Polyclonal Antibody | anti-DOK1 antibody

Dok1 (Tyr-362), phospho-specific

Cat. Num. AAA472020

• Gene Names: DOK1; P62DOK; MGC117395; MGC138860
• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified
• Synonyms: Dok1 (Tyr-362); Polyclonal Antibody; phospho-specific; p62DOK; anti-DOK1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was cross-adsorbed to a phosphotyrosine peptide before affinity purification using phospho-Dok1 (Tyr-362) peptide. The purified antibody detects a band at 62 kDa* corresponding to Dok1 in western blots of human Jurkat cells, but does not detect this band after alkaline phosphatase treatment. Similar to Dok1 (Ser-450) antibody, this antibody also detects an 80 kDa band that has not been identified.
• Purity/Purification: Affinity Purified
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-DOK1 antibody: ELISA, Western Blot
• Application Notes: ELISA: 1:2000; Western Blot: 1:1000
• Immunogen: Phospho-Dok1 (Tyr-362) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding tyrosine 362 in human Dok1. This sequence is conserved in Dok1 from rat and mouse (Tyr-361), and has high homology to Dok2 (Tyr-337). The site is not conserved in other Dok family members.
• Preparation and Storage: Store at -20 degree C. Do not aliquot. Stable for 1 year.

Testing Data

Related Product Information for anti-DOK1 antibody

Doks are a family of adaptor proteins that recruit SH2-containing molecules involved in various cell signaling pathways. Six Dok proteins (Dok1 to Dok6) have been identified and each has an N-terminal pleckstrin homology domain, a central phosphotyrosine binding domain, and a C-terminal region containing multiple tyrosine residues. When phosphorylated, these tyrosines can serve as docking sites for SH2 domain-containing proteins. Dok1 (p62dok) has been shown to bind Ras- GAP, Nck, and Csk. Several tyrosine phosphorylation sites have been identified for Dok1. One site, Tyr-362 (Tyr-361 mouse), is phosphorylated by c-Abl, is required for Nck binding, and may be critical for filopodia formation during fibroblast spreading on fibronectin. Alternatively, Dok1 activity is also regulated by serine phosphorylation. IkB Kinase b phosphorylates several serine sites including Ser-450 in vitro, and TNF-alpha, IL-1, and radiation treatment lead to phosphorylation of Ser-443, Ser-446, and Ser-450 in vivo. Phosphorylation of these serine sites may be required for Dok-mediated inhibition of MAPK signaling and stimulation of cell motility.

References

• Noguchi, T. et al. (1999) EMBOJ 18(7):1748.
• Kashige, N. et al. (2000) Proc. Nat. Acad. Sci. 97(5):2093.
• Lee, S. et al. (2004) Proc. Nat. Acad. Sci. 101(50):17416.
• Woodring, P.J. (2004) J Cell Biol. 165(4):493.

NCBI and Uniprot Product Information

• NCBI GI #: 4503357
• NCBI GeneID: 1796
• NCBI Accession #: NP_001372.1
• NCBI GenBank Nucleotide #: NM_001381.3
• UniProt Accession #: Q53GP8; Q14CB2; Q2TA81; Q99704
• Molecular Weight: 52,420 Da
• NCBI Official Full Name: docking protein 1 isoform 1
• NCBI Official Synonym Full Names: docking protein 1, 62kDa (downstream of tyrosine kinase 1)
• NCBI Official Symbol: DOK1
• NCBI Official Synonym Symbols: P62DOK; MGC117395; MGC138860
• NCBI Protein Information: docking protein 1; pp62; p62(dok); OTTHUMP00000160414; OTTHUMP00000202719; Downstream of tyrosine kinase 1; docking protein 1 (downstream of tyrosine kinase 1)
• UniProt Protein Name: Docking protein 1 variant
• Protein Family: Docking protein
• UniProt Entry Name: Q53GP8_HUMAN

NCBI Description

The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

DOK1: a docking protein that interacts with receptor tyrosine kinases. It is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; receptor signaling protein activity; insulin receptor binding

Biological Process: cell surface receptor linked signal transduction; Ras protein signal transduction; insulin receptor signaling pathway; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway