Rabbit Dok-2 Polyclonal Antibody | anti-Dok-2 antibody

Dok-2 (Phospho-Y299) Polyclonal Antibody

Cat. Num. AAA3005320

• Gene Names: DOK2; p56DOK; p56dok-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Dok-2; Polyclonal Antibody; Dok-2 (Phospho-Y299) Polyclonal Antibody; Docking protein 2; Downstream of tyrosine kinase 2; p56(dok-2); DOK2; anti-Dok-2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-Dok-2 (Y299) polyclonal antibody detects endogenous levels of Dok-2 protein when phosphorylated at Tyr299.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 412

• Applicable Applications for anti-Dok-2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human Dok-2 around the phosphorylation site of Tyrosine 299.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-Dok-2  (Y299)  pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with EGF (100ng/ml,30 minutes) whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug))

Related Product Information for anti-Dok-2 antibody

Dok-1 associates with the Ras GTPase activating protein (Ras GAP) upon tyrosine phosphorylation. Evidence suggests that p62 Dok-1 is a substrate of the constitutive tyrosine kinase activity of p210 Bcr-Abl, a fusion protein caused by the t(9;22) translocation and associated with chronic myelogenous leukemia. Dok-1, as well as the tyrosine kinase substrates IRS-1 and Cas, is a member of a class of "docking" proteins which contain multiple tyrosine residues and putative SH2 binding sites. Dok-1 is suspected to be the substrate phosphorylated in response to stimulation by a number of growth factors, including PDGF, VEGF, insulin and IGF. Dok-2 (also designated p56 Dok) has also been identified as a potential mediator of the effects of p210 Bcr-Abl.

NCBI and Uniprot Product Information

• NCBI GI #: 41406050
• NCBI GeneID: 9046
• NCBI Accession #: NP_003965.2
• NCBI GenBank Nucleotide #: NP_003965.2
• UniProt Accession #: O60496
• Molecular Weight: ~ 56kDa
• NCBI Official Full Name: docking protein 2 isoform a
• NCBI Official Synonym Full Names: docking protein 2
• NCBI Official Symbol: DOK2
• NCBI Official Synonym Symbols: p56DOK; p56dok-2
• NCBI Protein Information: docking protein 2
• UniProt Protein Name: Docking protein 2
• UniProt Gene Name: DOK2
• UniProt Entry Name: DOK2_HUMAN

NCBI Description

The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq, Jul 2008]

Uniprot Description

DOK2: a member of the p62dok family of proteins that interact with receptor tyrosine kinases and mediate particular biological responses. Constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/Abl), a chimeric protein whose presence is associated with CML. Contains a pleckstrin homology domain at the amino terminus, 13 potential tyrosine phosphorylation sites, and six PXXP SH3 recognition motifs. Binds p120 (RasGAP) from CML cells.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: cytosol

Molecular Function: receptor signaling protein activity; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding

Biological Process: cell surface receptor linked signal transduction; Ras protein signal transduction; blood coagulation; signal transduction; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway