Rabbit anti-Human DOC2A Polyclonal Antibody | anti-DOC2A antibody

DOC2A, ID (DOC2A, Double C2-like domain-containing protein alpha) (AP)

Cat. Num. AAA6293169

• Gene Names: DOC2A; Doc2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DOC2A; Polyclonal Antibody; ID (DOC2A; Double C2-like domain-containing protein alpha) (AP); Double C2-like domain-containing protein alpha; anti-DOC2A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-DOC2A antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DOC2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284-314 amino acids from the Central region of human DOC2A.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Flow Cytometry (FC/FACS)

(DOC2A Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(DOC2A Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DOC2A antibody detected the DOC2A protein (arrow).)

Immunohistochemistry (IHC)

(DOC2A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DOC2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-DOC2A antibody

There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release.

Product Categories/Family for anti-DOC2A antibody

Antibodies; Proteins

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Glessner, J.T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(23):10584-10589(2010). Guilmatre, A., et al. Arch. Gen. Psychiatry 66(9):947-956(2009). Kumar, R.A., et al. PLoS ONE 4 (2), E4582 (2009): Higashio, H., et al. J. Immunol. 180(7):4774-4784(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 47078261
• NCBI GeneID: 8448
• NCBI Accession #: NP_003577.2
• NCBI GenBank Nucleotide #: NM_003586.2
• UniProt Accession #: Q14183; Q6P4G4; Q7Z5G0; Q8IVX0; B4DEJ2; H3BNH6
• Molecular Weight: 18,384 Da
• NCBI Official Full Name: double C2-like domain-containing protein alpha
• NCBI Official Synonym Full Names: double C2 domain alpha
• NCBI Official Symbol: DOC2A
• NCBI Official Synonym Symbols: Doc2
• NCBI Protein Information: double C2-like domain-containing protein alpha
• UniProt Protein Name: Double C2-like domain-containing protein alpha
• Protein Family: Double C2-like domain-containing protein
• UniProt Gene Name: DOC2A
• UniProt Synonym Gene Names: Doc2; Doc2-alpha
• UniProt Entry Name: DOC2A_HUMAN

NCBI Description

There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]