Rabbit anti-Human DNASE1L3 Polyclonal Antibody | anti-DNASE1L3 antibody

DNASE1L3, NT (DNASE1L3, DHP2, DNAS1L3, Deoxyribonuclease gamma, DNase I homolog protein DHP2, Deoxyribonuclease I-like 3, Liver and spleen DNase) (Azide free) (HRP)

Cat. Num. AAA6292942

• Gene Names: DNASE1L3; LSD; DHP2; SLEB16; DNAS1L3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DNASE1L3; Polyclonal Antibody; NT (DNASE1L3; DHP2; DNAS1L3; Deoxyribonuclease gamma; DNase I homolog protein DHP2; Deoxyribonuclease I-like 3; Liver and spleen DNase) (Azide free) (HRP); Liver and spleen DNase; anti-DNASE1L3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DNASE1L3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DNASE1L3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 14-43 in the N-terminal region of human DNASE1L3, UniProt Accession #NP_004935.1; Q13609.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS644267.This demonstrates that MBS644267 detected the DNASE1L3 protein (arrow).)

Related Product Information for anti-DNASE1L3 antibody

This gene encodes a member of the DNase family. The protein hydrolyzes DNA, is not inhibited by actin, and mediates the breakdown of DNA during apoptosis. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Product Categories/Family for anti-DNASE1L3 antibody

Antibodies; Enzymes; Deoxyribonuclease

References

1. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 2. Ueki, M., et al. Clin. Chim. Acta 407 (1-2), 20-24 (2009). 3. Mizuta, R., et al. Biomed. Res. 30(3):165-170(2009). 4. Boulares, H., et al. Biochem. Biophys. Res. Commun. 341(2):653-662(2006). 5. Okamoto, M., et al. Biochem. Biophys. Res. Commun. 327(1):76-83(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4826698
• NCBI GeneID: 1776
• NCBI Accession #: NP_004935.1
• NCBI GenBank Nucleotide #: NM_004944.3
• UniProt Accession #: Q13609
• NCBI Official Full Name: deoxyribonuclease gamma isoform 1
• NCBI Official Synonym Full Names: deoxyribonuclease 1 like 3
• NCBI Official Symbol: DNASE1L3
• NCBI Official Synonym Symbols: LSD; DHP2; SLEB16; DNAS1L3
• NCBI Protein Information: deoxyribonuclease gamma
• UniProt Protein Name: Deoxyribonuclease gamma
• Protein Family: Deoxyribonuclease
• UniProt Gene Name: DNASE1L3
• UniProt Synonym Gene Names: DHP2; DNAS1L3; DNase gamma; DNase I-like 3; LS-DNase; LSD
• UniProt Entry Name: DNSL3_HUMAN

NCBI Description

This gene encodes a member of the deoxyribonuclease I family. The encoded protein hydrolyzes DNA, is not inhibited by actin, and mediates the breakdown of DNA during apoptosis. Mutations in this gene are a cause of systemic lupus erythematosus-16. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

DNASE1L3: Has DNA hydrolytic activity. Does not bind to actin. Cleaves chromatin DNA to nucleosomal units. Defects in DNASE1L3 are the cause of systemic lupus erythematosus type 16 (SLEB16). A rare autosomal recessive form of systemic lupus erythematosus with childhood onset, characterized by high frequency of anti-neutrophil cytoplasmic antibodies and lupus nephritis. Systemic lupus erythematosus is a chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow. Belongs to the DNase I family.

Protein type: EC 3.1.21.-; Secreted, signal peptide; Calcium-binding; Secreted; Deoxyribonuclease; Apoptosis

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: nucleus

Molecular Function: endodeoxyribonuclease activity, producing 5'-phosphomonoesters; DNA binding; deoxyribonuclease activity; calcium ion binding; endodeoxyribonuclease activity

Biological Process: DNA fragmentation during apoptosis; DNA metabolic process

Disease: Systemic Lupus Erythematosus 16