Rabbit anti-Human DNAJB2 Polyclonal Antibody | anti-DNAJB2 antibody

DNAJB2 Antibody

Cat. Num. AAA9602960

• Gene Names: DNAJB2; HSJ1; CMT2T; DSMA5; HSJ-1; HSPF3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: DNAJB2; Polyclonal Antibody; DNAJB2 Antibody; Descriptions; DnaJ (Hsp40) homolog subfamily B member 2; DnaJ homolog subfamily B member 2; DnaJ protein homolog 1; Heat shock 40 kDa protein 3; Heat shock protein J1; Heat shock protein neuronal DNAJ like 1; HSJ 1; HSJ1; HSPF3; anti-DNAJB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DNAJB2 antibody detects endogenous levels of total DNAJB2
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 277

• Applicable Applications for anti-DNAJB2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human DNAJB2
• Subcellular Location: Nucleus; Endoplasmic Reticulum
• Tissue Specificity: More abundantly expressed in neocortex, cerebellum, spinal cord and retina where it is expressed by neuronal cells (at protein level) (PubMed:1599432, PubMed:12754272). Detected at much lower level in non-neuronal tissues including kidney, lung, heart, skeletal muscle, spleen and testis (at protein level) (PubMed:12754272, PubMed:1599432). Isoform 1 is more abundant in neocortex and cerebellum compared to isoform 2 (at protein level) (PubMed:12754272).
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Dog
• Similarity: Pig (89%), Bovine (89%), Horse (89%), Sheep (89%), Dog (89%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of DNAJB2 using HUVEC whole cell lysates)

Related Product Information for anti-DNAJB2 antibody

Function: Functions as a co-chaperone, regulating the substrate binding and activating the ATPase activity of chaperones of the HSP70/heat shock protein 70 family (PubMed:7957263, PubMed:22219199). In parallel, also contributes to the ubiquitin-dependent proteasomal degradation of misfolded proteins (PubMed:15936278, PubMed:21625540). Thereby, may regulate the aggregation and promote the functional recovery of misfolded proteins like HTT, MC4R, PRKN, RHO and SOD1 and be crucial for many biological processes (PubMed:12754272, PubMed:20889486, PubMed:21719532, PubMed:22396390, PubMed:24023695). Isoform 1 which is localized to the endoplasmic reticulum membranes may specifically function in ER-associated protein degradation of misfolded proteins (PubMed:15936278).
Subunit Structure: Interacts with HSP70 (HSPA1A or HSPA1B) (PubMed:21625540, PubMed:22219199). Interacts with HSPA8/Hsc70 (PubMed:15936278). Interacts with PSMA3 and most probably with the whole proteasomal complex (PubMed:15936278).
Post-translational Modifications: Ubiquitinated by STUB1; does not lead to proteasomal degradation.
Similarity: The J domain is sufficient to interact with HSP70 (HSPA1A or HSPA1B) and activate its ATPase activity (PubMed:22219199). The J domain is also required for the HSP70-mediated and ubiquitin-dependent proteasomal degradation of proteins like ATXN3 (PubMed:21625540). The J domain is required to reduce PRKN cytoplasmic aggregation (PubMed:20889486).The UIM domains mediate interaction with ubiquitinated chaperone clients and with the proteasome (PubMed:15936278). The UIM domains may have an opposite activity to the J domain, binding ubiquitinated proteins and protecting them from HSP70-mediated proteasomal degradation (PubMed:21625540). The UIM domains are not required to reduce PRKN cytoplasmic aggregation (PubMed:20889486).

NCBI and Uniprot Product Information

• NCBI GI #: 88501736
• NCBI GeneID: 3300
• NCBI Accession #: NP_001034639.1
• NCBI GenBank Nucleotide #: NM_001039550.1
• UniProt Accession #: P25686; Q53QD7; Q8IUK1; Q8IUK2; Q96F52; A8K9P6
• Molecular Weight: Observed: 36 kDa; Predicted: 36 kDa
• NCBI Official Full Name: dnaJ homolog subfamily B member 2 isoform a
• NCBI Official Synonym Full Names: DnaJ heat shock protein family (Hsp40) member B2
• NCBI Official Symbol: DNAJB2
• NCBI Official Synonym Symbols: HSJ1; CMT2T; DSMA5; HSJ-1; HSPF3
• NCBI Protein Information: dnaJ homolog subfamily B member 2
• UniProt Protein Name: DnaJ homolog subfamily B member 2
• Protein Family: DnaJ homolog subfamily
• UniProt Gene Name: DNAJB2
• UniProt Synonym Gene Names: HSJ11 PublicationManual assertion based on opinion iniRef.1; HSJ-11 PublicationManual assertion based on opinion iniRef.1

NCBI Description

This gene is almost exclusively expressed in the brain, mainly in the neuronal layers. It encodes a protein that shows sequence similarity to bacterial DnaJ protein and the yeast homologs. In bacteria, this protein is implicated in protein folding and protein complex dissociation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

Functions as a co-chaperone, regulating the substrate binding and activating the ATPase activity of chaperones of the HSP70/heat shock protein 70 family (PubMed:7957263, PubMed:22219199). In parallel, also contributes to the ubiquitin-dependent proteasomal degradation of misfolded proteins (PubMed:15936278, PubMed:21625540). Thereby, may regulate the aggregation and promote the functional recovery of misfolded proteins like HTT, MC4R, PRKN, RHO and SOD1 and be crucial for many biological processes (PubMed:12754272, PubMed:20889486, PubMed:21719532, PubMed:22396390, PubMed:24023695). Isoform 1 which is localized to the endoplasmic reticulum membranes may specifically function in ER-associated protein degradation of misfolded proteins (PubMed:15936278).