Rabbit anti-Human DNAJA3 Polyclonal Antibody | anti-DNAJA3 antibody

DNAJA3, NT (DNAJA3, HCA57, TID1, DnaJ homolog subfamily A member 3, mitochondrial, DnaJ protein Tid-1, Hepatocellular carcinoma-associated antigen 57, Tumorous imaginal discs protein Tid56 homolog) (FITC)

Cat. Num. AAA6292622

• Gene Names: DNAJA3; TID1; HCA57; hTID-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DNAJA3; Polyclonal Antibody; NT (DNAJA3; HCA57; TID1; DnaJ homolog subfamily A member 3; mitochondrial; DnaJ protein Tid-1; Hepatocellular carcinoma-associated antigen 57; Tumorous imaginal discs protein Tid56 homolog) (FITC); Tumorous imaginal discs protein Tid56 homolog; anti-DNAJA3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DNAJA3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DNAJA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-134 amino acids from the N-terminal region of human DNAJA3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DNAJA3 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the DNAJA3 antibody detected the DNAJA3 protein (arrow).)

Related Product Information for anti-DNAJA3 antibody

DNAJA3 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins. For background information on the DNAJ family, see MIM 608375.

Product Categories/Family for anti-DNAJA3 antibody

Antibodies; Cancer Markers

References

Qian, J., et al. Gastroenterology 138(4):1418-1428(2010). Kurzik-Dumke, U., et al. J Transl Med 8, 58 (2010): Linnoila, J., et al. Neuron 60(4):625-641(2008). Kurzik-Dumke, U., et al. Int. J. Mol. Med. 21(1):19-31(2008). Kurzik-Dumke, U., et al. Cell. Signal. 19(9):1973-1985(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 205360840
• NCBI GeneID: 9093
• NCBI Accession #: NP_001128582.1; NP_005138.3
• NCBI GenBank Nucleotide #: NM_001135110.2
• UniProt Accession #: Q96EY1; O75472; Q8WUJ6; Q8WXJ3; Q96D76; Q96IV1; Q9NYH8; B2RAJ5
• Molecular Weight: 52,489 Da
• NCBI Official Full Name: dnaJ homolog subfamily A member 3, mitochondrial isoform 2
• NCBI Official Synonym Full Names: DnaJ (Hsp40) homolog, subfamily A, member 3
• NCBI Official Symbol: DNAJA3
• NCBI Official Synonym Symbols: TID1; HCA57; hTID-1
• NCBI Protein Information: dnaJ homolog subfamily A member 3, mitochondrial; dnaJ protein Tid-1; tumorous imaginal discs protein Tid56 homolog; hepatocellular carcinoma-associated antigen 57
• UniProt Protein Name: DnaJ homolog subfamily A member 3, mitochondrial
• Protein Family: DnaJ homolog subfamily
• UniProt Gene Name: DNAJA3
• UniProt Synonym Gene Names: HCA57; TID1; hTid-1

NCBI Description

This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.