Human Perforin/Pore-Forming Protein (PF/PFP) ELISA Kit | PF/PFP elisa kit

Human Perforin/Pore-Forming Protein (PF/PFP) ELISA Kit

Cat. Num. AAA3802377

• Gene Names: PRF1; P1; PFP; HPLH2
• Reactivity: Human
• Synonyms: Perforin/Pore-Forming Protein (PF/PFP); Human Perforin/Pore-Forming Protein (PF/PFP) ELISA Kit; PF/PFP elisa kit

• Reactivity: Human
• Sequence Length: 555

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 62.5 pg/ml-1000 pg/ml
• Sensitivity: 1 pg/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PF/PFP elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofPF/PFPin the sample, This PF/PFPELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusPF/PFPconcentration. The concentration ofPF/PFPin the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 133908621
• NCBI GeneID: 5551
• NCBI Accession #: NP_001076585.1
• NCBI GenBank Nucleotide #: NP_001076585.1
• UniProt Accession #: P14222
• NCBI Official Full Name: perforin-1
• NCBI Official Synonym Full Names: perforin 1
• NCBI Official Symbol: PRF1
• NCBI Official Synonym Symbols: P1; PFP; HPLH2
• NCBI Protein Information: perforin-1
• UniProt Protein Name: Perforin-1
• UniProt Gene Name: PRF1
• UniProt Synonym Gene Names: PFP; P1; PFP
• UniProt Entry Name: PERF_HUMAN

NCBI Description

This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]

Uniprot Description

PRF1: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Monomer, as sobluble protein. Homooligomer. Oligomerization is required for pore formation. Repressed by contact with target cells. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: membrane; cytoplasmic membrane-bound vesicle; integral to membrane; extracellular region; plasma membrane

Molecular Function: protein binding; calcium ion binding; wide pore channel activity

Biological Process: formation of immunological synapse; apoptosis; cytolysis; defense response to tumor cell; cellular defense response; immune response to tumor cell; defense response to virus; transmembrane transport; protein homooligomerization

Disease: Lymphoma, Non-hodgkin, Familial; Aplastic Anemia; Hemophagocytic Lymphohistiocytosis, Familial, 2