Rabbit anti-Human, Mouse DNA Primase Polyclonal Antibody | anti-dnaG antibody
DNA Primase (p49)
Gene Names
dnaG; dnaP; ECK3056; JW3038; parB; sdgA
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
DNA Primase; Polyclonal Antibody; DNA Primase (p49); Anti -DNA Primase (p49); anti-dnaG antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes the mouse p49 subunit at ~49kD. No reactivity is seen with p58 subunit. Species Crossreactivity: human
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.5,, 0.2% BSA 0.05% sodium azide before the addition of glycerol to 40%.
Applicable Applications for anti-dnaG antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1-5ug/ml
Dilution: Western Blot: 1-5ug/ml
Immunogen
Purified mouse recombinant DNA primase, p49.
Positive Control
HeLa cells or other tumor cells
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-dnaG antibody
In the eukaryotic cell, DNA primase initiates DNA replication by the synthesis of small ribonucleotides called primers. The eukaryotic primase is composed of two subunits, p49 (human 420aa; chromosome 12q13) and p58 (human 509aa, chromosome 6p12-p11.1), which purify as a complex tightly bound to DNA polymerase alpha. Primase initiates synthesis with a triphosphate purine moiety at the 5'-end. After synthesis of 7-10 ribonucleotides, the primer-template is translocated intramolecularly to the active site of the DNA polymerase alpha subunit. The p49 subunit of DNA primase contains the catalytic active site. Residue 104-111 are most critical for primer synthesis. Alanine substitution in residues Glu, Asp, and these residues may form part of a conserved carboxylic triad also observed in the active sites of DNA polymerases and reverse transcriptases.
Product Categories/Family for anti-dnaG antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,565 Da
NCBI Official Full Name
DNA primase
NCBI Official Symbol
dnaG
NCBI Official Synonym Symbols
dnaP; ECK3056; JW3038; parB; sdgA
NCBI Protein Information
DNA primase
UniProt Protein Name
DNA primase
Protein Family
UniProt Gene Name
dnaG
UniProt Synonym Gene Names
dnaP; parB
UniProt Entry Name
PRIM_ECOLI
NCBI Description
DNA primase catalyzes the synthesis of RNA primers on single-stranded template DNA . [More information is available at EcoCyc: EG10239].
Uniprot Description
Function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis.
Cofactor: Binds 1 zinc ion per monomer.
Subunit structure: Monomer.
Sequence similarities: Belongs to the DNA primase family.Contains 1 Toprim domain.
Sequence caution: The sequence CAA23531.1 differs from that shown. Reason: Frameshift at positions 106 and 169.
Research Articles on dnaG
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Product Notes
The dnaG dnag (Catalog #AAA613687) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNA Primase (p49) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DNA Primase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1-5ug/ml. Researchers should empirically determine the suitability of the dnaG dnag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Primase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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