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Western Blot (WB) (Western blot analysis of DNA Polymerase epsilon 2 expression in HL60 (A), HeLa (B) whole cell lysates.)

Rabbit DNA Polymerase epsilon 2 Polyclonal Antibody | anti-POLE2 antibody

Anti-DNA Polymerase epsilon 2 Antibody

Gene Names
POLE2; DPE2
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
DNA Polymerase epsilon 2; Polyclonal Antibody; Anti-DNA Polymerase epsilon 2 Antibody; DPE2; DNA polymerase epsilon subunit 2; DNA polymerase II subunit 2; DNA polymerase epsilon subunit B; anti-POLE2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of DNA Polymerase epsilon 2 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
501
Applicable Applications for anti-POLE2 antibody
Western Blot (WB)
Application Notes
WB (1/500 - 1/1000)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DNA Polymerase epsilon 2. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of DNA Polymerase epsilon 2 expression in HL60 (A), HeLa (B) whole cell lysates.)

Western Blot (WB) (Western blot analysis of DNA Polymerase epsilon 2 expression in HL60 (A), HeLa (B) whole cell lysates.)
Related Product Information for anti-POLE2 antibody
Rabbit polyclonal antibody to DNA Polymerase epsilon 2

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56,784 Da
NCBI Official Full Name
DNA polymerase epsilon subunit 2 isoform 2
NCBI Official Synonym Full Names
polymerase (DNA directed), epsilon 2, accessory subunit
NCBI Official Symbol
POLE2
NCBI Official Synonym Symbols
DPE2
NCBI Protein Information
DNA polymerase epsilon subunit 2; DNA polymerase II subunit 2; DNA polymerase epsilon subunit B; polymerase (DNA directed), epsilon 2 (p59 subunit)
UniProt Protein Name
DNA polymerase epsilon subunit 2
Protein Family
UniProt Gene Name
POLE2
UniProt Synonym Gene Names
DPE2
UniProt Entry Name
DPOE2_HUMAN

Uniprot Description

POLE2: Participates in DNA repair and in chromosomal DNA replication. Belongs to the DNA polymerase epsilon subunit B family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - pyrimidine; DNA repair, damage; Nucleotide Metabolism - purine; EC 2.7.7.7; Transferase; DNA replication

Chromosomal Location of Human Ortholog: 14q21-q22

Cellular Component: nucleoplasm; epsilon DNA polymerase complex; intracellular membrane-bound organelle

Molecular Function: DNA binding; DNA-directed DNA polymerase activity

Biological Process: telomere maintenance via semi-conservative replication; DNA replication initiation; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; error-prone postreplication DNA repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA-dependent DNA replication; DNA repair; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle

Research Articles on POLE2

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Product Notes

The POLE2 pole2 (Catalog #AAA821570) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DNA Polymerase epsilon 2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DNA Polymerase epsilon 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB (1/500 - 1/1000). Researchers should empirically determine the suitability of the POLE2 pole2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Polymerase epsilon 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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