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Western Blot (WB) (All lanes : Anti-DMRT2 Antibody (Center) at 1:2000 dilutionLane 1: human kidney lysateLane 2: human skeletal muscle lysateLane 3: human testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human DMRT2 Polyclonal Antibody | anti-DMRT2 antibody

DMRT2 Antibody (Center)

Reactivity
Human
Applications
Western Blot
Synonyms
DMRT2; Polyclonal Antibody; DMRT2 Antibody (Center); Purified Rabbit Polyclonal Antibody (Pab); Doublesex- and mab-3-related transcription factor 2; Doublesex-like 2 protein; DSXL-2; DSXL2; anti-DMRT2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This DMRT2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 357-392 amino acids from the Central region of human DMRT2.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
226
Applicable Applications for anti-DMRT2 antibody
Western Blot (WB)
Application Notes
WB ~~ 1:2000
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-DMRT2 Antibody (Center) at 1:2000 dilutionLane 1: human kidney lysateLane 2: human skeletal muscle lysateLane 3: human testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-DMRT2 Antibody (Center) at 1:2000 dilutionLane 1: human kidney lysateLane 2: human skeletal muscle lysateLane 3: human testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-DMRT2 antibody
Transcriptional activator that directly regulates early activation of the myogenic determination gene MYF5 by binding in a sequence-specific manner to the early epaxial enhancer element of it. Involved in somitogenesis during embryogenesis and somite development and differentiation into sclerotome and dermomyotome. Required for the initiation and/or maintenance of proper organization of the sclerotome, dermomyotome and myotome (By similarity).
Product Categories/Family for anti-DMRT2 antibody
References
Raymond C.S.,et al.Hum. Mol. Genet. 8:989-996(1999). Ottolenghi C.,et al.Genomics 64:179-186(2000). Calvari V.,et al.Genomics 65:203-212(2000). Humphray S.J.,et al.Nature 429:369-374(2004). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61814
NCBI Official Full Name
doublesex- and mab-3-related transcription factor 2 isoform 1
NCBI Official Synonym Full Names
doublesex and mab-3 related transcription factor 2
NCBI Official Symbol
DMRT2
NCBI Protein Information
doublesex- and mab-3-related transcription factor 2
UniProt Protein Name
Doublesex- and mab-3-related transcription factor 2
UniProt Gene Name
DMRT2
UniProt Synonym Gene Names
DSXL2; DSXL-2
UniProt Entry Name
DMRT2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the DMRT gene family, sharing a DM DNA-binding domain with Drosophila 'doublesex' (dsx) and C. elegans mab3, genes involved in sex determination in these organisms. Also, this gene is located in a region of the human genome (chromosome 9p24.3) associated with gonadal dysgenesis and XY sex reversal. Hence this gene is one of the candidates for sex-determining gene(s) on chr 9. [provided by RefSeq, Apr 2010]

Uniprot Description

DMRT2: belongs to the DMRT gene family, sharing a DM DNA-binding domain with Drosophila 'doublesex' (dsx) and C. elegans mab3, genes involved in sex determination in these organisms. Also, this gene is located in a region of the human genome (chromosome 9p24.3) associated with gonadal dysgenesis and XY sex reversal. Hence this gene is one of the candidates for sex-determining gene(s) on chr 9. [provided by RefSeq, Apr 2010]

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 9p24.3

Cellular Component: nucleus

Molecular Function: protein homodimerization activity; metal ion binding; transcription factor activity

Biological Process: transcription, DNA-dependent; embryonic skeletal development; positive regulation of transcription from RNA polymerase II promoter; regulation of somitogenesis; sex differentiation

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Product Notes

The DMRT2 dmrt2 (Catalog #AAA9215618) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DMRT2 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DMRT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:2000. Researchers should empirically determine the suitability of the DMRT2 dmrt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DMRT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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