Rabbit anti-Human; Mouse DLL1-Specific Polyclonal Antibody | anti-DLL1 antibody
DLL1-Specific Antibody
Gene Names
DLL1; DL1; Delta; DELTA1
Reactivity
Human; Mouse
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
DLL1-Specific; Polyclonal Antibody; DLL1-Specific Antibody; DLL1 Antibody - Internal; Delta (Drosophila) like 1; Delta; Delta like 1 (Drosophila); Delta like 1; Delta like 1 protein; Delta like protein 1; Delta like protein 1 precursor; Drosophila; homolog of 1; Delta-like protein 1; Delta1; DL1; DLL 1; DLL1; DLL1_HUMAN; Drosophila Delta homolog 1; H-Delta-1; OTTHUMP00000017690;; anti-DLL1 antibody
Host
Rabbit
Reactivity
Human; Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
DLL1-Specific Antibody detects endogenous levels of DLL1-Specific.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Sequence Length
723
Applicable Applications for anti-DLL1 antibody
Western Blot (WB)
Application Notes
WB 1:500-1:2000
Immunogen
A synthesized peptide derived from human DLL1-Specific.
Fragment
Fab fragment
Predicted Cross Reactivity
Conjugation
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C. Stable for 12 months from date of receipt.
Post Translational Modifications
Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation (By similarity). Ubiquitinated; promotes recycling back to the plasma membrane and confers a strong affinity for NOTCH1. Multi-ubiquitination of LYS-613 by MIB1 promotes both cis and trans-interaction with NOTCH1, as well as activation of Notch signaling. Ubiquitinated by NEURL1B (By similarity).Phosphorylated in a membrane association-dependent manner. Phosphorylation at Ser-697 requires the presence of Ser-694, whereas phosphorylation at Ser-694 occurs independently of the other site. Phosphorylation is required for full ligand activity in vitro and affects surface presentation, ectodomain shedding, and endocytosis.O-fucosylated. Can be elongated to a disaccharide by MFNG.
Preparation and Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 degree C. Stable for 12 months from date of receipt.
Related Product Information for anti-DLL1 antibody
Transmembrane ligand protein of NOTCH1, NOTCH2 and NOTCH3 receptors that binds the extracellular domain (ECD) of Notch receptor in a cis and trans fashion manner (PubMed:11006133). Following transinteraction, ligand cells produce mechanical force that depends of a clathrin-mediated endocytosis, requiring ligand ubiquitination, EPN1 interaction, and actin polymerisation; these events promote Notch receptor extracellular domain (NECD) transendocytosis and triggers Notch signaling through induction of cleavage, hyperphosphorylation, and nuclear accumulation of the intracellular domain of Notch receptors (NICD) (By similarity). Is required for embryonic development and maintenance of adult stem cells in many different tissues and immune systeme; the DLL1-induced Notch signaling is mediated through an intercellular communication that regulates cell lineage, cell specification, cell patterning and morphogenesis through effects on differentiation and proliferation (PubMed:11581320). Plays a role in brain development at different level, namely by regulating neuronal differentiation of neural precursor cells via cell-cell interaction, most likely through the lateral inhibitory system in an endogenous level dependent-manner. During neocortex development, Dll1-Notch signaling transmission is mediated by dynamic interactions between intermediate neurogenic progenitors and radial glia; the cell-cell interactions are mediated via dynamic and transient elongation processes, likely to reactivate/maintain Notch activity in neighboring progenitors, and coordinate progenitor cell division and differentiation across radial and zonal boundaries. During cerebellar development, regulates Bergmann glial monolayer formation and its morphological maturation through a Notch signaling pathway. At the retina and spinal cord level, regulates neurogenesis by preventing the premature differentiation of neural progenitors and also by maintaining progenitors in spinal cord through Notch signaling pathway. Also controls neurogenesis of the neural tube in a progenitor domain-specific fashion along the dorsoventral axis. Maintains quiescence of neural stem cells and plays a role as a fate determinant that segregates asymmetrically to one daughter cell during neural stem cells mitosis, resulting in neuronal differentiation in Dll1-inheriting cell. Plays a role in immune systeme development, namely the development of all T-cells and marginal zone (MZ) B-cells (By similarity). Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor (PubMed:11581320). Also plays a role during muscle development. During early development, inhibits myoblasts differentiation from the medial dermomyotomal lip and later regulates progenitor cell differentiation. Directly modulates cell adhesion and basal lamina formation in satellite cells through Notch signaling. Maintains myogenic progenitors pool by suppressing differentiation through down-regulation of MYOD1 and is required for satellite cell homing and PAX7 expression. During craniofacial and trunk myogenesis suppresses differentiation of cranial mesoderm-derived and somite-derived muscle via MYOD1 regulation but in cranial mesoderm-derived progenitors, is neither required for satellite cell homing nor for PAX7 expression. Also plays a role during pancreatic cell development. During type B pancreatic cell development, may be involved in the initiation of proximodistal patterning in the early pancreatic epithelium. Stimulates multipotent pancreatic progenitor cells proliferation and pancreatic growth by maintaining HES1 expression and PTF1A protein levels. During fetal stages of development, is required to maintain arterial identity and the responsiveness of arterial endothelial cells for VEGFA through regulation of KDR activation and NRP1 expression. Controls sprouting angiogenesis and subsequent vertical branch formation througth regulation on tip cell differentiation. Negatively regulates goblet cell differentiation in intestine and controls secretory fat commitment through lateral inhibition in small intestine. Plays a role during inner ear development; negatively regulates auditory hair cell differentiation. Plays a role during nephron development through Notch signaling pathway. Regulates growth, blood pressure and energy homeostasis (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Mol.Wt.: 80 kDa. Predicted Mol.Wt.: 79kDa.
NCBI Official Full Name
delta-like protein 1
NCBI Official Synonym Full Names
delta like canonical Notch ligand 1
NCBI Official Symbol
DLL1
NCBI Official Synonym Symbols
DL1; Delta; DELTA1
NCBI Protein Information
delta-like protein 1
UniProt Protein Name
Delta-like protein 1
Protein Family
UniProt Gene Name
DLL1
UniProt Synonym Gene Names
Delta1; H-Delta-1
UniProt Entry Name
DLL1_HUMAN
NCBI Description
DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. [provided by RefSeq, Jul 2008]
Uniprot Description
DLL1: Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. Interacts with Notch receptors. Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
Protein type: Cell development/differentiation; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 6q27
Cellular Component: integral to plasma membrane; extracellular region; plasma membrane; cytoplasmic vesicle
Molecular Function: protein binding; calcium ion binding; Notch binding
Biological Process: regulation of cell adhesion; Notch signaling pathway; negative regulation of myeloid cell differentiation; negative regulation of interleukin-10 production; Notch receptor processing; cell fate determination; compartment specification; somite specification; cell-cell signaling; negative regulation of auditory receptor cell differentiation; hemopoiesis; positive regulation of transcription from RNA polymerase II promoter; heart looping; determination of left/right symmetry; cell differentiation; inner ear development; positive regulation of Notch signaling pathway
Research Articles on DLL1
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Product Notes
The DLL1 dll1 (Catalog #AAA9611696) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DLL1-Specific Antibody reacts with Human; Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DLL1-Specific can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB 1:500-1:2000. Researchers should empirically determine the suitability of the DLL1 dll1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DLL1-Specific, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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