Immunofluorescence (IF)
(MBS9600243 staining MCF-7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit DLGP1 Polyclonal Antibody | anti-DLGP1 antibody
DLGP1 Antibody
Gene Names
DLGAP1; DAP1; GKAP; DAP-1; SAPAP1; DLGAP1A; DLGAP1B; DAP-1-BETA; DAP-1-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DLGP1; Polyclonal Antibody; DLGP1 Antibody; 4933422O14Rik; 9630002F18; AI845682; AI848168; BB075781; D17Bwg0511e; DAP 1 ALPHA; DAP 1; DAP 1 BETA; DAP-1; DAP-1-ALPHA; DAP-1-BETA; DAP1; Discs large (Drosophila) homolog associated protein 1; Discs large homolog associated protein 1; Disks large associated protein 1; alpha isoform; included; beta isoform; Disks large-associated protein 1; DLGAP 1; Dlgap1; DLGP1_HUMAN; GKAP; GKAP/SAPAP; GKPA/SAPAP; Guanylate kinase associated protein; Guanylate kinase-associated protein; hGKAP; KIAA4162; MGC88156; mKIAA4162; PSD 95/SAP90 binding protein 1; PSD-95/SAP90-binding protein 1; SAP90/PSD 95 associated protein 1; SAP90/PSD-95-associated protein 1; SAPAP 1; SAPAP1; anti-DLGP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DLGP1 antibody detects endogenous levels of total DLGP1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
675
Applicable Applications for anti-DLGP1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:3000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human DLGP1
Subcellular Location
Cell Membrane. Cell Junction > Synapse > Postsynaptic Cell Membrane > Postsynaptic Density. Cell Junction > Synapse. Found in postsynaptic density of neuronal cells.
Tissue Specificity
Expressed in brain.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9600243 staining MCF-7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9600243 staining MCF-7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis on HepG2 cell lysate using DLGP1 Antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis on HepG2 cell lysate using DLGP1 Antibody.The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-DLGP1 antibody
Description: SAPAP1 Part of the postsynaptic scaffold in neuronal cells. Belongs to the SAPAP family. Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN.
Function: Part of the postsynaptic scaffold in neuronal cells.
Subunit Structure: Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1 (By similarity). Interacts with MPP2 (via the SH3-Guanylate kinase-like sub-module) (By similarity).
Similarity: Belongs to the SAPAP family.
Function: Part of the postsynaptic scaffold in neuronal cells.
Subunit Structure: Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1 (By similarity). Interacts with MPP2 (via the SH3-Guanylate kinase-like sub-module) (By similarity).
Similarity: Belongs to the SAPAP family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 108 kDa
Predicted: 109 kDa
Predicted: 109 kDa
NCBI Official Full Name
disks large-associated protein 1 isoform 2
NCBI Official Synonym Full Names
DLG associated protein 1
NCBI Official Symbol
DLGAP1
NCBI Official Synonym Symbols
DAP1; GKAP; DAP-1; SAPAP1; DLGAP1A; DLGAP1B; DAP-1-BETA; DAP-1-ALPHA
NCBI Protein Information
disks large-associated protein 1
UniProt Protein Name
Disks large-associated protein 1
UniProt Gene Name
DLGAP1
UniProt Synonym Gene Names
DAP1; GKAP; DAP-1; hGKAP; SAPAP1
Uniprot Description
Part of the postsynaptic scaffold in neuronal cells.
Research Articles on DLGP1
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Product Notes
The DLGP1 dlgap1 (Catalog #AAA9600243) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DLGP1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DLGP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:3000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the DLGP1 dlgap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DLGP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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