Immunohistochemistry (IHC)
(Immunochemical staining of human DLEC1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human DLEC1 Polyclonal Antibody | anti-DLEC1 antibody
Anti-DLEC1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
DLEC1; Polyclonal Antibody; Anti-DLEC1 Antibody; Rabbit Polyclonal; DLEC1 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-DLC1 Antibody; Anti-F56 Antibody; anti-DLEC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human DLEC1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-DLEC1 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human DLEC1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human DLEC1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human DLEC1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human DLEC1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-DLEC1 antibody
The tumor suppressor DLEC1 promotes cell proliferation when AP-2alpha2 is down-regulated in HCT116 stable clones, suggesting its pro-survival nature. DLEC1 can be a pro-survival protein under certain circumstances and a potential therapeutic target for increasing sensitivity of cancer cells to 5-FU. DLEC1 inhibits carcinoma cell growth through inducing cell cycle arrest and apoptosis, and also suppresses cell metastasis by reversing epithelial-mesenchymal transition (EMT) and cell stemness. Moreover, DLEC1 represses oncogenic signaling including JAK/STAT3, MAPK/ERK, Wnt/beta-catenin and AKT pathways in multiple carcinoma types. DLEC1 contains three YXXQ motifs and forms a protein complex with STAT3 via protein docking, which blocks STAT3-JAK2 interaction and STAT3 phosphorylation. IL-6 stimulation enhances the binding of DLEC1 with STAT3, which diminishes their interaction with JAK2 and further leads to decreased STAT3 phosphorylation. The YXXQ motifs of DLEC1 are crucial for its inhibition of STAT3 phosphorylation, and disruption of these motifs restores STAT3 phosphorylation through abolishing DLEC1 binding to STAT3. DLEC1 methylation can be utilized to identify a subset with a better prognosis in intrahepatic cholangiocarcinomas of the small duct type.
References
Qiu GH, et al. (2019) The pro-survival function of dlec1 and its protection of cancer cells against 5-fu-induced apoptosis through up-regulation of bcl-xl. Cytotechnology 71 (1): 23-33.Li X, et al. (2019) Clinicopathological significance and diagnostic value of dlec1 hypermethylation in lung cancer: A meta-analysis. J Nippon Med Sch 86 (2): 62-69.Li L, et al. (2018) Epigenomic characterization of a p53-regulated 3p22.2 tumor suppressor that inhibits stat3 phosphorylation via protein docking and is frequently methylated in esophageal and other carcinomas. Theranostics 8 (1): 61-77.
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Product Notes
The DLEC1 (Catalog #AAA8110259) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DLEC1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DLEC1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the DLEC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DLEC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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