Rabbit anti-Human, Mouse Phkg1 Polyclonal Antibody | anti-Phkg1 antibody

Phkg1, CT (Phkg1, Phkg, Phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform, Phosphorylase kinase subunit gamma-1) (FITC)

Cat. Num. AAA6333390

• Gene Names: Phkg1; Phkg
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Phkg1; Polyclonal Antibody; CT (Phkg1; Phkg; Phosphorylase b kinase gamma catalytic chain; skeletal muscle isoform; Phosphorylase kinase subunit gamma-1) (FITC); Phosphorylase kinase subunit gamma-1; anti-Phkg1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Phkg1 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Phkg1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-331 amino acids from the C-terminal region of Mouse Phkg1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Phkg1 Antibody (C-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the Phkg1 antibody detected the Phkg1 protein (arrow).)

Western Blot (WB)

(Mouse Phkg1 Antibody (C-term) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the Phkg1 antibody detected the Phkg1 protein (arrow).)

Flow Cytometry (FC/FACS)

(Mouse Phkg1 Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-Phkg1 antibody

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I.

Product Categories/Family for anti-Phkg1 antibody

Antibodies; Protein Kinases

References

Suto, J., et al. J. Vet. Med. Sci. 66(9):1033-1038(2004). Visel, A., et al. Nucleic Acids Res. 32 (DATABASE ISSUE), D552-D556 (2004): Takenaka, M., et al. Kidney Int. 53(3):562-572(1998). Maichele, A.J., et al. Mamm. Genome 5(1):15-18(1994) Bender, P.K., et al. Genet. Anal. Tech. Appl. 10 (3-4), 99-101 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 6755054
• NCBI GeneID: 18682
• NCBI Accession #: NP_035209.1
• NCBI GenBank Nucleotide #: NM_011079.2
• UniProt Accession #: P07934
• Molecular Weight: 44,960 Da
• NCBI Official Full Name: phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
• NCBI Official Synonym Full Names: phosphorylase kinase gamma 1
• NCBI Official Symbol: Phkg1
• NCBI Official Synonym Symbols: Phkg
• NCBI Protein Information: phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform; phosphorylase kinase subunit gamma-1; serine/threonine-protein kinase PHKG1; Phosphorylase B kinase gamma catalytic chain, skeletal muscle isoform (Phosphorylase kinase gamma sub
• UniProt Protein Name: Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
• Protein Family: Phosphorylase b kinase
• UniProt Gene Name: Phkg1
• UniProt Synonym Gene Names: Phkg
• UniProt Entry Name: PHKG1_MOUSE