Rabbit anti-Human Diubiquitin Polyclonal Antibody | anti-UBD antibody

Anti-Diubiquitin antibody

Cat. Num. AAA176811

• Gene Names: UBD; FAT10; UBD-3; GABBR1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Diubiquitin; Polyclonal Antibody; Anti-Diubiquitin antibody; Ubiquitin D; ubiquitin D; Diubiquitin antibody; FAT10 antibody; UBD 3 antibody; Ubd antibody; UBD_HUMAN antibody; Ubiquitin D antibody; Ubiquitin like protein FAT10 antibody; Ubiquitin-like protein FAT10 antibody; anti-UBD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 165

• Applicable Applications for anti-UBD antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Diubiquitin (27-40aa YDSVKKIKEHVRSK), different from the related mouse and rat sequences by five amino acids.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-Diubiquitin antibody, MBS176811, Western blottingLane 1: HELA Cell LysateLane 2: SKOV Cell LysateLane 3: MCF-7 Cell LysateLane 4: A549 Cell LysateLane 5: SMMC Cell Lysate )

Immunohistochemistry (IHC)

(Anti-Diubiquitin antibody, MBS176811, IHC(P)IHC(P): Human Tonsil Tissue )

Immunohistochemistry (IHC)

(Anti-Diubiquitin antibody, MBS176811, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Related Product Information for anti-UBD antibody

Description: Rabbit IgG polyclonal antibody for Ubiquitin D(UBD) detection. Tested with WB, IHC-P in Human.
Background: Ubiquitin D, also called UBD or FAT10 is a protein that in humans is encoded by the UBD gene. This gene is mapped to 6p22.1. Ubiquitin-like protein modifier can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome in a NUB1L-dependent manner. This gene may be involved in dendritic cell(DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. It may be involved in the formation of aggresomes when proteasome is saturated or impaired. This gene mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus(HIV)-associated nephropathy(HIVAN). It probably functions as a survival factor.

NCBI and Uniprot Product Information

• NCBI GI #: 269849476
• NCBI GeneID: 10537
• UniProt Accession #: O15205; Q5STL2; Q5SUK2; Q96EC7; B0UZT6
• Molecular Weight: 18,473 Da
• NCBI Official Full Name: Ubiquitin D
• NCBI Official Synonym Full Names: ubiquitin D
• NCBI Official Symbol: UBD
• NCBI Official Synonym Symbols: FAT10; UBD-3; GABBR1
• NCBI Protein Information: ubiquitin D; diubiquitin; ubiquitin-like protein FAT10
• UniProt Protein Name: Ubiquitin D
• Protein Family: Ubiquitin
• UniProt Gene Name: UBD
• UniProt Synonym Gene Names: FAT10
• UniProt Entry Name: UBD_HUMAN

Uniprot Description

FAT10: Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. Probably functions as a survival factor. Conjugation ability activated by UBA6. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomes when proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)- associated nephropathy (HIVAN).

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein modification by small protein conjugation; positive regulation of apoptosis; protein ubiquitination; myeloid dendritic cell differentiation; proteolysis; response to organic nitrogen