Rabbit anti-Human DISC1 Polyclonal Antibody | anti-DISC1 antibody

DISC1, CT (DISC1, KIAA0457, Disrupted in schizophrenia 1 protein) (PE)

Cat. Num. AAA6292244

• Gene Names: DISC1; SCZD9; C1orf136
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DISC1; Polyclonal Antibody; CT (DISC1; KIAA0457; Disrupted in schizophrenia 1 protein) (PE); Disrupted in schizophrenia 1 protein; anti-DISC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 369

• Applicable Applications for anti-DISC1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DISC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 700-728 amino acids from the C-terminal region of human DISC1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DISC1 Antibody (C-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the DISC1 antibody detected the DISC1 protein (arrow).)

Flow Cytometry (FC/FACS)

(DISC1 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(DISC1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DISC1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-DISC1 antibody

This gene encodes a protein with multiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its interaction with other proteins. This gene is disrupted in a t(1;11)(q42.1;q14.3) translocation which segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Product Categories/Family for anti-DISC1 antibody

Antibodies; KIAA; orf

References

Park, Y.U., et al. Proc. Natl. Acad. Sci. U.S.A. 107(41):17785-17790(2010). Raznahan, A., et al. Mol. Psychiatry (2010). In press: Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). Kaibuchi, K., et al. Nihon Shinkei Seishin Yakurigaku Zasshi 30(3):149-152(2010). Shulman, J.M., et al. PLoS ONE 5 (6), E11244 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 61742827
• NCBI GeneID: 27185
• NCBI Accession #: NP_001012976.1; NP_061132.2
• NCBI GenBank Nucleotide #: NP_001012976.1
• UniProt Accession #: Q9NRI5
• NCBI Official Full Name: disrupted in schizophrenia 1 protein isoform Es
• NCBI Official Synonym Full Names: DISC1 scaffold protein
• NCBI Official Symbol: DISC1
• NCBI Official Synonym Symbols: SCZD9; C1orf136
• NCBI Protein Information: disrupted in schizophrenia 1 protein
• UniProt Protein Name: Disrupted in schizophrenia 1 protein
• Protein Family: Disrupted in schizophrenia
• UniProt Gene Name: DISC1
• UniProt Synonym Gene Names: KIAA0457
• UniProt Entry Name: DISC1_HUMAN

NCBI Description

This gene encodes a protein with multiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its interaction with other proteins. This gene is disrupted in a t(1;11)(q42.1;q14.3) translocation which segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

DISC1: Involved in the regulation of multiple aspects of embryonic and adult neurogenesis. Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance. Plays a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. Inhibits the activation of AKT-mTOR signaling upon interaction with CCDC88A. Regulates the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Plays a role, together with PCNT, in the microtubule network formation. A chromosomal aberration involving DISC1 segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Translocation t(1;11)(q42.1;q14.3). The truncated DISC1 protein produced by this translocation is unable to interact with ATF4, ATF5 and NDEL1. Genetic variation in DISC1 is associated with susceptibility to schizophrenia type 9 (SCZD9). A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q42.1

Cellular Component: microtubule; centrosome; postsynaptic membrane; mitochondrion; postsynaptic density; cell junction

Molecular Function: protein binding

Biological Process: positive regulation of neuroblast proliferation; mitochondrial calcium ion homeostasis; TOR signaling pathway; cell proliferation in forebrain; neuron migration; cerebral cortex radially oriented cell migration; Wnt receptor signaling pathway through beta-catenin; microtubule cytoskeleton organization and biogenesis; positive regulation of Wnt receptor signaling pathway

Disease: Schizophrenia; Schizophrenia 9